Seq-Well: Portable, low-cost rna sequencing of single cells at high throughput

Single-cell rna-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. here, we present seq-Well, a portable, low-cost platform for massively parallel single-cell rna-seq. barcoded mrna capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling effcient cell lysis and transcript capture. We use seq-Well to profle thousands of primary human macrophages exposed to Mycobacterium tuberculosis.