Seq-Well: Portable, low-cost rna sequencing of single cells at high throughput

Todd M. Gierahn, Marc H. Wadsworth, Travis K. Hughes, Bryan D. Bryson, Andrew Butler, Rahul Satija, Sarah Fortune, J. Christopher Love, Alex K. Shalek

Research output: Contribution to journalArticle

Abstract

Single-cell rna-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. here, we present seq-Well, a portable, low-cost platform for massively parallel single-cell rna-seq. barcoded mrna capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling effcient cell lysis and transcript capture. We use seq-Well to profle thousands of primary human macrophages exposed to Mycobacterium tuberculosis.

Original languageEnglish (US)
Pages (from-to)395-398
Number of pages4
JournalNature methods
Volume14
Issue number4
DOIs
StatePublished - Feb 13 2017

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Gierahn, T. M., Wadsworth, M. H., Hughes, T. K., Bryson, B. D., Butler, A., Satija, R., Fortune, S., Christopher Love, J., & Shalek, A. K. (2017). Seq-Well: Portable, low-cost rna sequencing of single cells at high throughput. Nature methods, 14(4), 395-398. https://doi.org/10.1038/nmeth.4179