TY - JOUR
T1 - Sequence analysis of closely related retrotransposon families from fission yeast
AU - Weaver, Daniel C.
AU - Shpakovski, George V.
AU - Caputo, Emerita
AU - Levin, Henry L.
AU - Bocke, J. D.
N1 - Funding Information:
G.V.S. thanks Drs. H.A. Nash (Laboratory of Molecular Biology) and M.B. Brennan (Unit on Genomics) for encouragement and advice during his stay at NIMH. G.V.S. is on leave from Institute of Bioorganic Chemistry, Academy of Sciences of Belarus, Minsk. We thank Daniel Voytas for helpful discussions. Supported in part by a Howard Hughes Undergraduate Summer Research Fellowship (D.C.W.); a Fogarty International Fellowship and CEEI grant (G.V.S.); a postdoctoral fellowship from the American Cancer Society (H.L.L.); Public Health Service training grant CA09139 from the National Institutes of Health, Public Health Service grant CA16519 from the National Institutes of Health, and American Cancer Society Faculty research award FRA-366 (J.D.B.).
PY - 1993/9/6
Y1 - 1993/9/6
N2 - Two families of retrotransposons, Tf1 and Tf2, have been isolated from the fission yeast, Schizosaccharomyces pombe. We report here the nucleotide (nt) sequence of a Tf2 element, the only retrotransposon family known from the commonly used laboratory strains, 972 and 975, and their derivatives. The total nt sequence of Tf2 was derived from the complete sequence of the coding region and 3′ long terminal repeat (LTR) of randomly cloned element Tf2-1, and from a full 5′ LTR and approximately one-third of the open reading frame (ORF) of Tf2-43, a Tf2 element found in the head-to-head orientation adjacent to the Sz. pombe rpb6 gene. The two Tf2 sequences are nearly identical and both of them contain a single ORF encoding a protein with regions of sequence similar to protease, reverse transcriptase, RNase H (RH) and integrase from other retrotransposons and retroviruses. Sequence comparisons between Tf1 and Tf 2 indicate an extreme divergence of the putative capsid protein-encoding regions of these two elements, as well as divergence of a segment of the LTR, but otherwise virtually identical sequence.
AB - Two families of retrotransposons, Tf1 and Tf2, have been isolated from the fission yeast, Schizosaccharomyces pombe. We report here the nucleotide (nt) sequence of a Tf2 element, the only retrotransposon family known from the commonly used laboratory strains, 972 and 975, and their derivatives. The total nt sequence of Tf2 was derived from the complete sequence of the coding region and 3′ long terminal repeat (LTR) of randomly cloned element Tf2-1, and from a full 5′ LTR and approximately one-third of the open reading frame (ORF) of Tf2-43, a Tf2 element found in the head-to-head orientation adjacent to the Sz. pombe rpb6 gene. The two Tf2 sequences are nearly identical and both of them contain a single ORF encoding a protein with regions of sequence similar to protease, reverse transcriptase, RNase H (RH) and integrase from other retrotransposons and retroviruses. Sequence comparisons between Tf1 and Tf 2 indicate an extreme divergence of the putative capsid protein-encoding regions of these two elements, as well as divergence of a segment of the LTR, but otherwise virtually identical sequence.
KW - Schizosaccharomyces pombe
KW - Tf2 element
KW - complete nucleotide sequence
KW - evolution
KW - rpb6 gene
KW - transposable elements
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U2 - 10.1016/0378-1119(93)90682-S
DO - 10.1016/0378-1119(93)90682-S
M3 - Article
C2 - 8396547
AN - SCOPUS:0027213993
SN - 0378-1119
VL - 131
SP - 135
EP - 139
JO - Gene
JF - Gene
IS - 1
ER -