Sequence-specific backbone NMR assignments for the C-terminal globular domain of EMILIN-1 [1]

Guiliana Verdone; Simon A. Colebrooke; Jonathan Boyd; Paolo Viglino; Alessandra Corazza; Roberto Doliana; Gabriella Mungiguerra; Alfonso Colombatti; Gennaro Esposito; Iain D. Campbell

doi:10.1023/B:JNMR.0000019460.94913.6a

Sequence-specific backbone NMR assignments for the C-terminal globular domain of EMILIN-1 [1]

Guiliana Verdone, Simon A. Colebrooke, Jonathan Boyd, Paolo Viglino, Alessandra Corazza, Roberto Doliana, Gabriella Mungiguerra, Alfonso Colombatti, Gennaro Esposito, Iain D. Campbell

Chemistry

Research output: Contribution to journal › Letter › peer-review

Original language	English (US)
Pages (from-to)	91-92
Number of pages	2
Journal	Journal of Biomolecular NMR
Volume	29
Issue number	1
DOIs	https://doi.org/10.1023/B:JNMR.0000019460.94913.6a
State	Published - May 2004

Keywords

C1q-like domain
EMILIN
Elastic fibres
Heteronuclear NMR
Partially deuterated proteins
TROSY
Triple resonance experiments

ASJC Scopus subject areas

Biochemistry
Spectroscopy

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10.1023/B:JNMR.0000019460.94913.6a

Cite this

@article{e0ce9ce6d4ee4610a35556493c8f4b9c,

title = "Sequence-specific backbone NMR assignments for the C-terminal globular domain of EMILIN-1 [1]",

keywords = "C1q-like domain, EMILIN, Elastic fibres, Heteronuclear NMR, Partially deuterated proteins, TROSY, Triple resonance experiments",

author = "Guiliana Verdone and Colebrooke, {Simon A.} and Jonathan Boyd and Paolo Viglino and Alessandra Corazza and Roberto Doliana and Gabriella Mungiguerra and Alfonso Colombatti and Gennaro Esposito and Campbell, {Iain D.}",

note = "Funding Information: The chemical shift information obtained for the 13Cα, 13Cβ and 13C′ nuclei was used to identify secondary structure elements in the protein according to the CSI method (Wishart and Sykes, 1994). Based on this analysis, eleven segments of β strands could be identified. These secondary structure elements in the EMILIN-1 C1q domain closely match the β strand pattern observed in the crystal structures of the two homologous domains from ACRP-30 and Collagen X (Shapiro and Scherer, 1998; Bogin et al., 2002).1H, 13C and 15N chemical shifts have been deposited in the BioMagResBank (http://www.bmrb.wisc.edu) under the BMRB accession number 5882. Acknowledgements The authors would like to thank the Italian Ministry of Education (FIRB, COFIN), EMBO and JIF, for funding. Helpful discussions with Dr C.D. Blundell and the kind assistance of Dr A. Makek are gratefully acknowledged. References Bartels, C., Xia, T.H., Billeter, M., Guntert, P. and W{\"u}thrich, K. (1995) J. Biomol. NMR, 6: 1–10. Bogin, O., Kvansakul, M., Rom, E., Singer, J., Yayon, A. and Hohenester, E. (2002) Structure, 10: 165–173. Doliana, R., Mongiat, M., Bucciotti, F., Giacomello, E., Deutzmann, R., Volpin, D., Bressan, G.M. and Colombatti, A. (1999) J. Biol. Chem., 274: 16773–16781. Mongiat, M., Mungiguerra, G., Bot, S., Mucignat, M.T., Giac-omello, E., Doliana, R. and Colombatti, A. (2000) J. Biol. Chem., 275: 25471–25480. Pervushin, K., Riek, R., Wider, G. and W{\"u}thrich, K. (1997) Proc. Natl. Acad. Sci. USA, 94: 12366–12371. Salzmann, M., Wider, G., Pervushin, K., Senn, H. and Wuthrich, K. (1999) J. Am. Chem. Soc., 121: 844–848. Shapiro, L. and Scherer, P.E. (1998) Curr. Biol., 8: 335–338. Spessotto, P., Cervi, M., Mucignat, M.T., Mungiguerra, G., Sartor-etto, I., Doliana, R. and Colombatti, A. (2003) J. Biol. Chem., 278: 6160–6167. Wishart, D.S. and Sykes, B.D. (1994) J. Biol. NMR, 4: 171–180. Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L. and Sykes, B.D. (1995) J. Biomol. NMR, 6: 135–140.",

year = "2004",

month = may,

doi = "10.1023/B:JNMR.0000019460.94913.6a",

language = "English (US)",

volume = "29",

pages = "91--92",

journal = "Journal of Biomolecular NMR",

issn = "0925-2738",

publisher = "Springer Science and Business Media B.V.",

number = "1",

}

TY - JOUR

T1 - Sequence-specific backbone NMR assignments for the C-terminal globular domain of EMILIN-1 [1]

AU - Verdone, Guiliana

AU - Colebrooke, Simon A.

AU - Boyd, Jonathan

AU - Viglino, Paolo

AU - Corazza, Alessandra

AU - Doliana, Roberto

AU - Mungiguerra, Gabriella

AU - Colombatti, Alfonso

AU - Esposito, Gennaro

AU - Campbell, Iain D.

N1 - Funding Information: The chemical shift information obtained for the 13Cα, 13Cβ and 13C′ nuclei was used to identify secondary structure elements in the protein according to the CSI method (Wishart and Sykes, 1994). Based on this analysis, eleven segments of β strands could be identified. These secondary structure elements in the EMILIN-1 C1q domain closely match the β strand pattern observed in the crystal structures of the two homologous domains from ACRP-30 and Collagen X (Shapiro and Scherer, 1998; Bogin et al., 2002).1H, 13C and 15N chemical shifts have been deposited in the BioMagResBank (http://www.bmrb.wisc.edu) under the BMRB accession number 5882. Acknowledgements The authors would like to thank the Italian Ministry of Education (FIRB, COFIN), EMBO and JIF, for funding. Helpful discussions with Dr C.D. Blundell and the kind assistance of Dr A. Makek are gratefully acknowledged. References Bartels, C., Xia, T.H., Billeter, M., Guntert, P. and Wüthrich, K. (1995) J. Biomol. NMR, 6: 1–10. Bogin, O., Kvansakul, M., Rom, E., Singer, J., Yayon, A. and Hohenester, E. (2002) Structure, 10: 165–173. Doliana, R., Mongiat, M., Bucciotti, F., Giacomello, E., Deutzmann, R., Volpin, D., Bressan, G.M. and Colombatti, A. (1999) J. Biol. Chem., 274: 16773–16781. Mongiat, M., Mungiguerra, G., Bot, S., Mucignat, M.T., Giac-omello, E., Doliana, R. and Colombatti, A. (2000) J. Biol. Chem., 275: 25471–25480. Pervushin, K., Riek, R., Wider, G. and Wüthrich, K. (1997) Proc. Natl. Acad. Sci. USA, 94: 12366–12371. Salzmann, M., Wider, G., Pervushin, K., Senn, H. and Wuthrich, K. (1999) J. Am. Chem. Soc., 121: 844–848. Shapiro, L. and Scherer, P.E. (1998) Curr. Biol., 8: 335–338. Spessotto, P., Cervi, M., Mucignat, M.T., Mungiguerra, G., Sartor-etto, I., Doliana, R. and Colombatti, A. (2003) J. Biol. Chem., 278: 6160–6167. Wishart, D.S. and Sykes, B.D. (1994) J. Biol. NMR, 4: 171–180. Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L. and Sykes, B.D. (1995) J. Biomol. NMR, 6: 135–140.

PY - 2004/5

Y1 - 2004/5

KW - C1q-like domain

KW - EMILIN

KW - Elastic fibres

KW - Heteronuclear NMR

KW - Partially deuterated proteins

KW - TROSY

KW - Triple resonance experiments

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UR - http://www.scopus.com/inward/citedby.url?scp=12144286351&partnerID=8YFLogxK

U2 - 10.1023/B:JNMR.0000019460.94913.6a

DO - 10.1023/B:JNMR.0000019460.94913.6a

M3 - Letter

C2 - 15017143

AN - SCOPUS:12144286351

SN - 0925-2738

VL - 29

SP - 91

EP - 92

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 1

ER -

Sequence-specific backbone NMR assignments for the C-terminal globular domain of EMILIN-1 [1]

Keywords

ASJC Scopus subject areas

Access to Document

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