TY - JOUR
T1 - Serologic differentiation between antitoxin responses to infection with Vibrio cholerae and enterotoxin-producing Escherichia coli
AU - Svennerholm, Ann Marl
AU - Holmgren, Jan
AU - Black, Robert
AU - Levine, Myron
AU - Merson, Michael
N1 - Funding Information:
This research was supported by grant no. 506B601 from the National Defense Research Institute in Sweden, project no. 16X-3382 from the Swedish Medical Research Council, funds from the International Centre for Diarrhoeal Disease Research, Bangladesh, contracts no. N01AI 42553 and no. N01AI 12666from the National Institute of Allergyand Infectious Diseases, and contract no. DAMD 17-78-c-8011 from the U.S. Army Medical Research and Development Command.
PY - 1983
Y1 - 1983
N2 - A ganglioside enzyme-linked immunosorbent assay (ELISA) was used to study and attempt to differentiate between antitoxin responses in persons infected with either Vibrio cholerae or Escherichia coli producing heat-labile enterotoxin. In most cases (69%-94%), experimentally infected North Americans and naturally infected Bangladeshis responded to either infection with significant (greater than twofold) increases in serum antibody titer to both heat-labile enterotoxin and cholera toxin. In all but one instance, the response was higher to the homologous than to the heterologous toxin, and for the Americans the homologous antitoxin titers remained significantly higher for at least one year. Determination of levels of antibodies to purified subunits A and B of cholera toxin by an ELISA showed that V. cholerae infection in most instances induced a significant response to subunit B but rarely to subunit A. E. coli infection, on the other hand, induced only slight increases in antibody titer to either subunit.
AB - A ganglioside enzyme-linked immunosorbent assay (ELISA) was used to study and attempt to differentiate between antitoxin responses in persons infected with either Vibrio cholerae or Escherichia coli producing heat-labile enterotoxin. In most cases (69%-94%), experimentally infected North Americans and naturally infected Bangladeshis responded to either infection with significant (greater than twofold) increases in serum antibody titer to both heat-labile enterotoxin and cholera toxin. In all but one instance, the response was higher to the homologous than to the heterologous toxin, and for the Americans the homologous antitoxin titers remained significantly higher for at least one year. Determination of levels of antibodies to purified subunits A and B of cholera toxin by an ELISA showed that V. cholerae infection in most instances induced a significant response to subunit B but rarely to subunit A. E. coli infection, on the other hand, induced only slight increases in antibody titer to either subunit.
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U2 - 10.1093/infdis/147.3.514
DO - 10.1093/infdis/147.3.514
M3 - Article
AN - SCOPUS:0020655530
SN - 0304-3975
VL - 147
SP - 514
EP - 522
JO - Unknown Journal
JF - Unknown Journal
IS - 3
ER -