Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100β antisense gene

S. Ueda; E. T Kokotos Leonardi; J. Bell; E. C. Azmitia

doi:10.1016/0169-328X(94)00269-K

Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100β antisense gene

S. Ueda, E. T Kokotos Leonardi, J. Bell, E. C. Azmitia

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

S-100β, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100β in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100β antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100β from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.

Original language	English (US)
Pages (from-to)	365-368
Number of pages	4
Journal	Molecular Brain Research
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/0169-328X(94)00269-K
State	Published - Apr 1995

Keywords

Hippocampus
Neurotrophic factor
Transfection

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/0169-328X(94)00269-K

Cite this

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title = "Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100β antisense gene",

abstract = "S-100β, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100β in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100β antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100β from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.",

keywords = "Hippocampus, Neurotrophic factor, Transfection",

author = "S. Ueda and Leonardi, {E. T Kokotos} and J. Bell and Azmitia, {E. C.}",

note = "Funding Information: We would like to thank Dr. Linda Van Eldik for kindly supplying the C-6 glioma with the S-100/3 antisense and to Drs. Whitaker-Azmitia and Van Eldik for critical reading of the manuscript. Support was obtained from the NYU Technology Transfer Fund and National Institute of Aging Program Project (AG10208).",

year = "1995",

month = apr,

doi = "10.1016/0169-328X(94)00269-K",

language = "English (US)",

volume = "29",

pages = "365--368",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100β antisense gene

AU - Ueda, S.

AU - Leonardi, E. T Kokotos

AU - Bell, J.

AU - Azmitia, E. C.

N1 - Funding Information: We would like to thank Dr. Linda Van Eldik for kindly supplying the C-6 glioma with the S-100/3 antisense and to Drs. Whitaker-Azmitia and Van Eldik for critical reading of the manuscript. Support was obtained from the NYU Technology Transfer Fund and National Institute of Aging Program Project (AG10208).

PY - 1995/4

Y1 - 1995/4

N2 - S-100β, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100β in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100β antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100β from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.

AB - S-100β, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100β in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100β antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100β from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.

KW - Hippocampus

KW - Neurotrophic factor

KW - Transfection

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AN - SCOPUS:0028898369

SN - 0169-328X

VL - 29

SP - 365

EP - 368

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 2

ER -

Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100β antisense gene

Abstract

Keywords

ASJC Scopus subject areas

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