TY - JOUR
T1 - Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100β antisense gene
AU - Ueda, S.
AU - Leonardi, E. T Kokotos
AU - Bell, J.
AU - Azmitia, E. C.
N1 - Funding Information:
We would like to thank Dr. Linda Van Eldik for kindly supplying the C-6 glioma with the S-100/3 antisense and to Drs. Whitaker-Azmitia and Van Eldik for critical reading of the manuscript. Support was obtained from the NYU Technology Transfer Fund and National Institute of Aging Program Project (AG10208).
PY - 1995/4
Y1 - 1995/4
N2 - S-100β, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100β in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100β antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100β from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.
AB - S-100β, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100β in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100β antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100β from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.
KW - Hippocampus
KW - Neurotrophic factor
KW - Transfection
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U2 - 10.1016/0169-328X(94)00269-K
DO - 10.1016/0169-328X(94)00269-K
M3 - Article
C2 - 7609624
AN - SCOPUS:0028898369
SN - 0169-328X
VL - 29
SP - 365
EP - 368
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -