S-100β, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100β in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100β antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100β from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.
- Neurotrophic factor
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience