Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100β antisense gene

S. Ueda, E. T Kokotos Leonardi, J. Bell, E. C. Azmitia

Research output: Contribution to journalArticlepeer-review

Abstract

S-100β, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100β in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100β antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100β from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.

Original languageEnglish (US)
Pages (from-to)365-368
Number of pages4
JournalMolecular Brain Research
Volume29
Issue number2
DOIs
StatePublished - Apr 1995

Keywords

  • Hippocampus
  • Neurotrophic factor
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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