Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene

S. Ueda, E. T. Kokotos Leonardi, 3rd Bell J., E. C. Azmitia

Research output: Contribution to journalArticlepeer-review


S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.
Original languageUndefined
Pages (from-to)365-8
JournalMolecular Brain Research
Issue number2
StatePublished - 1995


  • Animals Calcium-Binding Proteins/genetics Cell Transplantation *DNA, Antisense Female Gene Expression Regulation, Neoplastic/*physiology Glioma/*genetics Neoplasm Transplantation Nerve Fibers/physiology Nerve Growth Factors/*genetics *Nerve Regeneration Rats Rats, Sprague-Dawley S100 Calcium Binding Protein beta Subunit S100 Proteins/genetics Serotonin/*physiology

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