Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene

S. Ueda; E. T. Kokotos Leonardi; 3rd Bell J.; E. C. Azmitia

Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene

S. Ueda, E. T. Kokotos Leonardi, 3rd Bell J., E. C. Azmitia

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.

Original language	Undefined
Pages (from-to)	365-8
Journal	Molecular Brain Research
Volume	29
Issue number	2
State	Published - 1995

Keywords

Animals Calcium-Binding Proteins/genetics Cell Transplantation *DNA, Antisense Female Gene Expression Regulation, Neoplastic/*physiology Glioma/*genetics Neoplasm Transplantation Nerve Fibers/physiology Nerve Growth Factors/*genetics *Nerve Regeneration Rats Rats, Sprague-Dawley S100 Calcium Binding Protein beta Subunit S100 Proteins/genetics Serotonin/*physiology

Cite this

@article{b3db89b25b814e3ba954f03f5f4664af,

title = "Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene",

abstract = "S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.",

keywords = "Animals Calcium-Binding Proteins/genetics Cell Transplantation *DNA, Antisense Female Gene Expression Regulation, Neoplastic/*physiology Glioma/*genetics Neoplasm Transplantation Nerve Fibers/physiology Nerve Growth Factors/*genetics *Nerve Regeneration Rats Rats, Sprague-Dawley S100 Calcium Binding Protein beta Subunit S100 Proteins/genetics Serotonin/*physiology",

author = "S. Ueda and {Kokotos Leonardi}, {E. T.} and {Bell J.}, 3rd and Azmitia, {E. C.}",

note = "Ueda, S Kokotos Leonardi, E T Bell, J 3rd Azmitia, E C AG10208/AG/NIA NIH HHS/United States Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Netherlands Brain Res Mol Brain Res. 1995 Apr;29(2):365-8.",

year = "1995",

language = "Undefined",

volume = "29",

pages = "365--8",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene

AU - Ueda, S.

AU - Kokotos Leonardi, E. T.

AU - Bell J., 3rd

AU - Azmitia, E. C.

N1 - Ueda, S Kokotos Leonardi, E T Bell, J 3rd Azmitia, E C AG10208/AG/NIA NIH HHS/United States Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Netherlands Brain Res Mol Brain Res. 1995 Apr;29(2):365-8.

PY - 1995

Y1 - 1995

N2 - S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.

AB - S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.

KW - Animals Calcium-Binding Proteins/genetics Cell Transplantation DNA, Antisense Female Gene Expression Regulation, Neoplastic/physiology Glioma/genetics Neoplasm Transplantation Nerve Fibers/physiology Nerve Growth Factors/genetics Nerve Regeneration Rats R

M3 - Article

SN - 0169-328X

VL - 29

SP - 365

EP - 368

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 2

ER -