Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation

David A. Baker; Joanne Thompson; Olalekan O. Daramola; Jane M R Carlton; Geoffrey A T Targett

doi:10.1016/0166-6851(95)00073-A

Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation

David A. Baker, Joanne Thompson, Olalekan O. Daramola, Jane M R Carlton, Geoffrey A T Targett

Research output: Contribution to journal › Article › peer-review

Abstract

A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.

Original language	English (US)
Pages (from-to)	193-201
Number of pages	9
Journal	Molecular and Biochemical Parasitology
Volume	72
Issue number	1-2
DOIs	https://doi.org/10.1016/0166-6851(95)00073-A
State	Published - Jun 1995

Keywords

Gametocyte
In situ hybridization
Malaria
Plasmodium falciparum

ASJC Scopus subject areas

Parasitology
Molecular Biology

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10.1016/0166-6851(95)00073-A

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title = "Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation",

abstract = "A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.",

keywords = "Gametocyte, In situ hybridization, Malaria, Plasmodium falciparum",

author = "Baker, {David A.} and Joanne Thompson and Daramola, {Olalekan O.} and Carlton, {Jane M R} and Targett, {Geoffrey A T}",

note = "Funding Information: We thank Mr. N. Rogers, Dr. D. Carucci, Mr. C. Drakeley, Dr. J. Kelly, Dr. C.S. Ong, Dr. B. Hall, Dr. A.G.-M.I. Lulat and Prof. R.E. Sinden for helpful discussions concerning this work. We are grateful for financial support from the Wellcome Trust. J.T. is supportedb y a grant from the European Commission Research Program STD3 to Prof. R.E. Sinden, and the Medical ResearchC ouncil. J.C. is supported by a grant from the Medical Research Council to Prof. D. Walliker.",

year = "1995",

month = jun,

doi = "10.1016/0166-6851(95)00073-A",

language = "English (US)",

volume = "72",

pages = "193--201",

journal = "Molecular and Biochemical Parasitology",

issn = "0166-6851",

publisher = "Elsevier B.V.",

number = "1-2",

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T1 - Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation

AU - Baker, David A.

AU - Thompson, Joanne

AU - Daramola, Olalekan O.

AU - Carlton, Jane M R

AU - Targett, Geoffrey A T

N1 - Funding Information: We thank Mr. N. Rogers, Dr. D. Carucci, Mr. C. Drakeley, Dr. J. Kelly, Dr. C.S. Ong, Dr. B. Hall, Dr. A.G.-M.I. Lulat and Prof. R.E. Sinden for helpful discussions concerning this work. We are grateful for financial support from the Wellcome Trust. J.T. is supportedb y a grant from the European Commission Research Program STD3 to Prof. R.E. Sinden, and the Medical ResearchC ouncil. J.C. is supported by a grant from the Medical Research Council to Prof. D. Walliker.

PY - 1995/6

Y1 - 1995/6

N2 - A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.

AB - A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.

KW - Gametocyte

KW - In situ hybridization

KW - Malaria

KW - Plasmodium falciparum

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Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation

Abstract

Keywords

ASJC Scopus subject areas

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