TY - JOUR
T1 - Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation
AU - Baker, David A.
AU - Thompson, Joanne
AU - Daramola, Olalekan O.
AU - Carlton, Jane M R
AU - Targett, Geoffrey A T
N1 - Funding Information:
We thank Mr. N. Rogers, Dr. D. Carucci, Mr. C. Drakeley, Dr. J. Kelly, Dr. C.S. Ong, Dr. B. Hall, Dr. A.G.-M.I. Lulat and Prof. R.E. Sinden for helpful discussions concerning this work. We are grateful for financial support from the Wellcome Trust. J.T. is supportedb y a grant from the European Commission Research Program STD3 to Prof. R.E. Sinden, and the Medical ResearchC ouncil. J.C. is supported by a grant from the Medical Research Council to Prof. D. Walliker.
PY - 1995/6
Y1 - 1995/6
N2 - A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.
AB - A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.
KW - Gametocyte
KW - In situ hybridization
KW - Malaria
KW - Plasmodium falciparum
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U2 - 10.1016/0166-6851(95)00073-A
DO - 10.1016/0166-6851(95)00073-A
M3 - Article
C2 - 8538689
AN - SCOPUS:0029045587
SN - 0166-6851
VL - 72
SP - 193
EP - 201
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1-2
ER -