Short communication: Identification of conformationally different binding sites in benzo(a)pyrene diol epoxide-dna adducts by low-temperature fluorescence spectroscopy

Seog K. Kim, Nicholas E. Geacintov, Henry C. Brenner, Ronald G. Harvey

Research output: Contribution to journalArticle

Abstract

It is known that the covalent binding of the two enantiomers of trans-7,8-diol-anti-9,10-epoxy-benzo[a]yrene (BPDE) to native double-stranded DNA gives rise to two distinct classes of adducts. Type I adducts involve significant interactions of the pyrenyl residues with the DNA bases and are similar but not identical to intercalation complexes. Type II adducts involve external solvent-exposed binding sites and their predominance in adducts derived from the covalent reaction of (+)-BPDE with DNA has been associated with the higher tumorigenicity and mutagenic activity of (+)-BPDE in mammalian cells. These two distinct binding sites in covalent BPDE-DNA adducts can be readily resolved by synchronous scanning fluorescence methods at low temperatures (77 K) using commercially available fluorescence spectrophoto-meters. The site I adducts are particularly unstable in the presence of UV light, and this method can be used to follow their selective photodegradation.

Original languageEnglish (US)
Pages (from-to)1333-1335
Number of pages3
JournalCarcinogenesis
Volume10
Issue number7
DOIs
StatePublished - Jul 1989

ASJC Scopus subject areas

  • Cancer Research

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