TY - JOUR
T1 - Short Photoswitchable Ceramides Enable Optical Control of Apoptosis
AU - Morstein, Johannes
AU - Kol, Matthijs
AU - Novak, Alexander J.E.
AU - Feng, Suihan
AU - Khayyo, Shadi
AU - Hinnah, Konstantin
AU - Li-Purcell, Nasi
AU - Pan, Grace
AU - Williams, Benjamin M.
AU - Riezman, Howard
AU - Atilla-Gokcumen, G. Ekin
AU - Holthuis, Joost C.M.
AU - Trauner, Dirk
N1 - Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/3/19
Y1 - 2021/3/19
N2 - We report short ceramide analogs that can be activated with light and further functionalized using azide-alkyne click chemistry. These molecules, termed scaCers, exhibit increased cell permeability compared to their long-chain analogs as demonstrated using mass spectrometry and imaging. Notably, scaCers enable optical control of apoptosis, which is not observed with long-chain variants. Additionally, they function as photoswitchable substrates for sphingomyelin synthase 2 (SMS2), exhibiting inverted light-dependence compared to their extended analogs.
AB - We report short ceramide analogs that can be activated with light and further functionalized using azide-alkyne click chemistry. These molecules, termed scaCers, exhibit increased cell permeability compared to their long-chain analogs as demonstrated using mass spectrometry and imaging. Notably, scaCers enable optical control of apoptosis, which is not observed with long-chain variants. Additionally, they function as photoswitchable substrates for sphingomyelin synthase 2 (SMS2), exhibiting inverted light-dependence compared to their extended analogs.
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U2 - 10.1021/acschembio.0c00823
DO - 10.1021/acschembio.0c00823
M3 - Article
C2 - 33586946
AN - SCOPUS:85101594381
SN - 1554-8929
VL - 16
SP - 452
EP - 456
JO - ACS Chemical Biology
JF - ACS Chemical Biology
IS - 3
ER -