@article{2a0402640d39414e891ea66b2936a783,
title = "Signal enhancement in protein NMR using the spin-noise tuning optimum",
abstract = "We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the optimal tuning condition, which may be offset by several 100 kHz from the conventional tuning settings using the noise response of the water protons as an indicator. Although the radio frequency-pulse durations are typically longer under such conditions, signal-to-noise gains of up to 22% were achieved. At salt concentrations up to 100 mM a substantial sensitivity gain was observed.",
keywords = "Absorbed circuit noise, Probe tuning, SNTO, Sensitivity, Signal-to-noise gain, Spin-noise",
author = "Martin Nausner and Michael Goger and Eli Bendet-Taicher and Judith Schlagnitweit and Alexej Jerschow and Norbert M{\"u}ller",
note = "Funding Information: Acknowledgments This work was supported by the Austrian Marshall Plan Foundation (to Nausner M), the FWF (Austrian Science Funds) Project No. P19635-N17 (to M{\"u}ller N), a grant (to Jerschow A) by the US NSF (CHE-0550054). We acknowledge inspiring discussions with Bain AD, Bermel W, Desvaux H, Itin B, Otting G, and Sleator T. The NMR facilities of the New York Structural Biology Center were used in this work. These facilities are supported by the New York State Office of Science, Technology, and Academic Research, and the National Institutes of Health (grant P41 FM66354). Funding Information: This work was presented first at the joint EUROMAR 2010 and 17th ISMAR Conference (July 4–9, 2010, Florence Italy) supported by the European Science Foundation (ESF) as part of the EMAR programme.",
year = "2010",
month = nov,
doi = "10.1007/s10858-010-9446-4",
language = "English (US)",
volume = "48",
pages = "157--167",
journal = "Journal of Biomolecular NMR",
issn = "0925-2738",
publisher = "Springer Netherlands",
number = "3",
}