Simultaneous epitope and transcriptome measurement in single cells

Marlon Stoeckius, Christoph Hafemeister, William Stephenson, Brian Houck-Loomis, Pratip K. Chattopadhyay, Harold Swerdlow, Rahul Satija, Peter Smibert

Research output: Contribution to journalArticlepeer-review

Abstract

High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels. Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements into an efficient, single-cell readout. CITE-seq is compatible with existing single-cell sequencing approaches and scales readily with throughput increases.

Original languageEnglish (US)
Pages (from-to)865-868
Number of pages4
JournalNature methods
Volume14
Issue number9
DOIs
StatePublished - Aug 31 2017

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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