Simultaneous false-colour imaging of birefringence, extinction and transmittance at camera speed

W. Kaminsky, E. Gunn, R. Sours, B. Kahr

Research output: Contribution to journalArticlepeer-review

Abstract

A polarized light imaging technique is introduced that simultaneously captures and unfolds transmittance, fast/slow axes directions and birefringence or linear dichroism from single camera exposures. The technique, based on the rotating polarizer method, is capable of sampling an object through polarizers at four different angles in a single image with the use of an image multiplexer. The range of possible applications is demonstrated with examples from the fields of biology, medicine, forensics and material sciences.

Original languageEnglish (US)
Pages (from-to)153-164
Number of pages12
JournalJournal of Microscopy
Volume228
Issue number2
DOIs
StatePublished - Nov 2007

Keywords

  • Amyloid plaque
  • Birefringence
  • Cholesteryl acetate
  • Crystal quality assessment
  • Dichroism
  • Electro-optic effect
  • Extinction
  • Human hair
  • Optical polarimetry
  • Polarized light
  • Water flea

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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