TY - JOUR
T1 - Single-cell delineation of lineage and genetic identity in the mouse brain
AU - Bandler, Rachel C.
AU - Vitali, Ilaria
AU - Delgado, Ryan N.
AU - Ho, May C.
AU - Dvoretskova, Elena
AU - Ibarra Molinas, Josue S.
AU - Frazel, Paul W.
AU - Mohammadkhani, Maesoumeh
AU - Machold, Robert
AU - Maedler, Sophia
AU - Liddelow, Shane A.
AU - Nowakowski, Tomasz J.
AU - Fishell, Gord
AU - Mayer, Christian
N1 - Publisher Copyright:
© 2021, The Author(s).
PY - 2022/1/20
Y1 - 2022/1/20
N2 - During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia1,2. The link between developmental lineage and cell-type diversity remains an open question. Here we used massively parallel tagging of progenitors to track clonal relationships and transcriptomic signatures during mouse forebrain development. We quantified clonal divergence and convergence across all major cell classes postnatally, and found diverse types of GABAergic neuron that share a common lineage. Divergence of GABAergic clones occurred during embryogenesis upon cell-cycle exit, suggesting that differentiation into subtypes is initiated as a lineage-dependent process at the progenitor cell level.
AB - During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia1,2. The link between developmental lineage and cell-type diversity remains an open question. Here we used massively parallel tagging of progenitors to track clonal relationships and transcriptomic signatures during mouse forebrain development. We quantified clonal divergence and convergence across all major cell classes postnatally, and found diverse types of GABAergic neuron that share a common lineage. Divergence of GABAergic clones occurred during embryogenesis upon cell-cycle exit, suggesting that differentiation into subtypes is initiated as a lineage-dependent process at the progenitor cell level.
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UR - http://www.scopus.com/inward/citedby.url?scp=85121366823&partnerID=8YFLogxK
U2 - 10.1038/s41586-021-04237-0
DO - 10.1038/s41586-021-04237-0
M3 - Article
C2 - 34912118
AN - SCOPUS:85121366823
SN - 0028-0836
VL - 601
SP - 404
EP - 409
JO - Nature
JF - Nature
IS - 7893
ER -