Single-cell delineation of lineage and genetic identity in the mouse brain

Rachel C. Bandler, Ilaria Vitali, Ryan N. Delgado, May C. Ho, Elena Dvoretskova, Josue S. Ibarra Molinas, Paul W. Frazel, Maesoumeh Mohammadkhani, Robert Machold, Sophia Maedler, Shane A. Liddelow, Tomasz J. Nowakowski, Gord Fishell, Christian Mayer

Research output: Contribution to journalArticlepeer-review


During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia1,2. The link between developmental lineage and cell-type diversity remains an open question. Here we used massively parallel tagging of progenitors to track clonal relationships and transcriptomic signatures during mouse forebrain development. We quantified clonal divergence and convergence across all major cell classes postnatally, and found diverse types of GABAergic neuron that share a common lineage. Divergence of GABAergic clones occurred during embryogenesis upon cell-cycle exit, suggesting that differentiation into subtypes is initiated as a lineage-dependent process at the progenitor cell level.

Original languageEnglish (US)
Pages (from-to)404-409
Number of pages6
Issue number7893
StatePublished - Jan 20 2022

ASJC Scopus subject areas

  • General


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