TY - JOUR
T1 - Single-channel activity induced in mitoplasts by alkaline pH
AU - Antonenko, Yuri N.
AU - Smith, David
AU - Kinnally, Kathleen W.
AU - Tedeschi, Henry
N1 - Funding Information:
Aided by NSF grant MCB9117658. We thank Charles Bowman for discussing with us various aspects of this article.
PY - 1994/9/14
Y1 - 1994/9/14
N2 - Exposure of patch-clamped mitoplasts to alkaline pH induces a reversible conductance increase (Antonenko, Yu. N., Kinnally, K.W. and Tedeschi, H. (1991) J. Membr. Biol. 124, 151-158) which is due to an increase in open probability of a channel activity of 15 pS and larger transitions. The present study defines in more detail some of the characteristics of the channel activity involved in this conductance increase. The results suggest the presence of two channels one slightly cation-selective of approx. 15 pS (referred to here as alkaline-induced cation-selective activity, ACA) and another slightly anion selective of approx. 45 pS (referred to as alkaline-induced anion-selective activity, AAA). The possible implication of these results in relation to other channels and the permeability transitions reported by others using mitochondrial suspensions is discussed.
AB - Exposure of patch-clamped mitoplasts to alkaline pH induces a reversible conductance increase (Antonenko, Yu. N., Kinnally, K.W. and Tedeschi, H. (1991) J. Membr. Biol. 124, 151-158) which is due to an increase in open probability of a channel activity of 15 pS and larger transitions. The present study defines in more detail some of the characteristics of the channel activity involved in this conductance increase. The results suggest the presence of two channels one slightly cation-selective of approx. 15 pS (referred to here as alkaline-induced cation-selective activity, ACA) and another slightly anion selective of approx. 45 pS (referred to as alkaline-induced anion-selective activity, AAA). The possible implication of these results in relation to other channels and the permeability transitions reported by others using mitochondrial suspensions is discussed.
KW - (Mitoplast)
KW - Alkaline-induced ion-selective activity
KW - Mitochondrial channel
KW - Mitochondrial inner membrane
KW - Patch clamp
KW - Permeability
KW - Single-channel activity
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U2 - 10.1016/0005-2736(94)90306-9
DO - 10.1016/0005-2736(94)90306-9
M3 - Article
C2 - 7522563
AN - SCOPUS:0028025157
SN - 0005-2736
VL - 1194
SP - 247
EP - 254
JO - BBA - Biomembranes
JF - BBA - Biomembranes
IS - 2
ER -