Single-channel activity induced in mitoplasts by alkaline pH

Yuri N. Antonenko; David Smith; Kathleen W. Kinnally; Henry Tedeschi

doi:10.1016/0005-2736(94)90306-9

Single-channel activity induced in mitoplasts by alkaline pH

Yuri N. Antonenko, David Smith, Kathleen W. Kinnally, Henry Tedeschi

Research output: Contribution to journal › Article › peer-review

Abstract

Exposure of patch-clamped mitoplasts to alkaline pH induces a reversible conductance increase (Antonenko, Yu. N., Kinnally, K.W. and Tedeschi, H. (1991) J. Membr. Biol. 124, 151-158) which is due to an increase in open probability of a channel activity of 15 pS and larger transitions. The present study defines in more detail some of the characteristics of the channel activity involved in this conductance increase. The results suggest the presence of two channels one slightly cation-selective of approx. 15 pS (referred to here as alkaline-induced cation-selective activity, ACA) and another slightly anion selective of approx. 45 pS (referred to as alkaline-induced anion-selective activity, AAA). The possible implication of these results in relation to other channels and the permeability transitions reported by others using mitochondrial suspensions is discussed.

Original language	English (US)
Pages (from-to)	247-254
Number of pages	8
Journal	BBA - Biomembranes
Volume	1194
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(94)90306-9
State	Published - Sep 14 1994

Keywords

(Mitoplast)
Alkaline-induced ion-selective activity
Mitochondrial channel
Mitochondrial inner membrane
Patch clamp
Permeability
Single-channel activity

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/0005-2736(94)90306-9

Cite this

@article{8e3f9ab7b6a54bf88fca71e1c1b6447b,

title = "Single-channel activity induced in mitoplasts by alkaline pH",

abstract = "Exposure of patch-clamped mitoplasts to alkaline pH induces a reversible conductance increase (Antonenko, Yu. N., Kinnally, K.W. and Tedeschi, H. (1991) J. Membr. Biol. 124, 151-158) which is due to an increase in open probability of a channel activity of 15 pS and larger transitions. The present study defines in more detail some of the characteristics of the channel activity involved in this conductance increase. The results suggest the presence of two channels one slightly cation-selective of approx. 15 pS (referred to here as alkaline-induced cation-selective activity, ACA) and another slightly anion selective of approx. 45 pS (referred to as alkaline-induced anion-selective activity, AAA). The possible implication of these results in relation to other channels and the permeability transitions reported by others using mitochondrial suspensions is discussed.",

keywords = "(Mitoplast), Alkaline-induced ion-selective activity, Mitochondrial channel, Mitochondrial inner membrane, Patch clamp, Permeability, Single-channel activity",

author = "Antonenko, {Yuri N.} and David Smith and Kinnally, {Kathleen W.} and Henry Tedeschi",

note = "Funding Information: Aided by NSF grant MCB9117658. We thank Charles Bowman for discussing with us various aspects of this article.",

year = "1994",

month = sep,

day = "14",

doi = "10.1016/0005-2736(94)90306-9",

language = "English (US)",

volume = "1194",

pages = "247--254",

journal = "BBA - Biomembranes",

issn = "0005-2736",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Single-channel activity induced in mitoplasts by alkaline pH

AU - Antonenko, Yuri N.

AU - Smith, David

AU - Kinnally, Kathleen W.

AU - Tedeschi, Henry

N1 - Funding Information: Aided by NSF grant MCB9117658. We thank Charles Bowman for discussing with us various aspects of this article.

PY - 1994/9/14

Y1 - 1994/9/14

N2 - Exposure of patch-clamped mitoplasts to alkaline pH induces a reversible conductance increase (Antonenko, Yu. N., Kinnally, K.W. and Tedeschi, H. (1991) J. Membr. Biol. 124, 151-158) which is due to an increase in open probability of a channel activity of 15 pS and larger transitions. The present study defines in more detail some of the characteristics of the channel activity involved in this conductance increase. The results suggest the presence of two channels one slightly cation-selective of approx. 15 pS (referred to here as alkaline-induced cation-selective activity, ACA) and another slightly anion selective of approx. 45 pS (referred to as alkaline-induced anion-selective activity, AAA). The possible implication of these results in relation to other channels and the permeability transitions reported by others using mitochondrial suspensions is discussed.

AB - Exposure of patch-clamped mitoplasts to alkaline pH induces a reversible conductance increase (Antonenko, Yu. N., Kinnally, K.W. and Tedeschi, H. (1991) J. Membr. Biol. 124, 151-158) which is due to an increase in open probability of a channel activity of 15 pS and larger transitions. The present study defines in more detail some of the characteristics of the channel activity involved in this conductance increase. The results suggest the presence of two channels one slightly cation-selective of approx. 15 pS (referred to here as alkaline-induced cation-selective activity, ACA) and another slightly anion selective of approx. 45 pS (referred to as alkaline-induced anion-selective activity, AAA). The possible implication of these results in relation to other channels and the permeability transitions reported by others using mitochondrial suspensions is discussed.

KW - (Mitoplast)

KW - Alkaline-induced ion-selective activity

KW - Mitochondrial channel

KW - Mitochondrial inner membrane

KW - Patch clamp

KW - Permeability

KW - Single-channel activity

UR - http://www.scopus.com/inward/record.url?scp=0028025157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028025157&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(94)90306-9

DO - 10.1016/0005-2736(94)90306-9

M3 - Article

C2 - 7522563

AN - SCOPUS:0028025157

SN - 0005-2736

VL - 1194

SP - 247

EP - 254

JO - BBA - Biomembranes

JF - BBA - Biomembranes

IS - 2

ER -

Single-channel activity induced in mitoplasts by alkaline pH

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this