Abstract
Laser-excited fluorescence from electrodynamically levitated microdroplets Is used to detect small numbers of rhodamlne-6G molecules. The small sample volume, typically a few plcollters reduces the background due to solvent and Impurity Raman and fluorescence emission. With 514.5-nm excitation from an argon Ion laser, as few as 12 molecules have been detected In glycerol-water droplets. Our present detection limit, due to variations In the Impurity concentration In the blanks, corresponds to a slgnal-to-nolse ratio of 3 for a single molecule of rhodamlne-6G contained In a 1-pL volume (dro plet diameter of ≃12 µm).
Original language | English (US) |
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Pages (from-to) | 1027-1031 |
Number of pages | 5 |
Journal | Analytical Chemistry |
Volume | 63 |
Issue number | 10 |
DOIs | |
State | Published - Jan 1991 |
ASJC Scopus subject areas
- Analytical Chemistry