Single virus detection from the reactive shift of a whispering-gallery mode

F. Vollmer; S. Arnold; D. Keng

doi:10.1073/pnas.0808988106

Single virus detection from the reactive shift of a whispering-gallery mode

F. Vollmer, S. Arnold, D. Keng

Research output: Contribution to journal › Article › peer-review

Abstract

We report the label-free, real-time optical detection of Influenza A virus particles. Binding of single virions is observed from discrete changes in the resonance frequency/wavelength of a whispering-gallery mode excited in a microspherical cavity. We find that the magnitude of the discrete wavelength-shift signal can be sufficiently enhanced by reducing the microsphere size. A reactive sensing mechanism with inverse dependence on mode volume is confirmed in experiments with virus-sized polystyrene nanoparticles. By comparing the electromagnetic theory for this reactive effect with experiments, the size and mass (≈5.2 × 10^-16 g) of a bound virion are determined directly from the optimal resonance shift.

Original language	English (US)
Pages (from-to)	20701-20704
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	105
Issue number	52
DOIs	https://doi.org/10.1073/pnas.0808988106
State	Published - Dec 30 2008

Keywords

Biosensor
Influenza
Optical resonance

ASJC Scopus subject areas

General

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10.1073/pnas.0808988106

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abstract = "We report the label-free, real-time optical detection of Influenza A virus particles. Binding of single virions is observed from discrete changes in the resonance frequency/wavelength of a whispering-gallery mode excited in a microspherical cavity. We find that the magnitude of the discrete wavelength-shift signal can be sufficiently enhanced by reducing the microsphere size. A reactive sensing mechanism with inverse dependence on mode volume is confirmed in experiments with virus-sized polystyrene nanoparticles. By comparing the electromagnetic theory for this reactive effect with experiments, the size and mass (≈5.2 × 10-16 g) of a bound virion are determined directly from the optimal resonance shift.",

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AU - Vollmer, F.

AU - Arnold, S.

AU - Keng, D.

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Y1 - 2008/12/30

N2 - We report the label-free, real-time optical detection of Influenza A virus particles. Binding of single virions is observed from discrete changes in the resonance frequency/wavelength of a whispering-gallery mode excited in a microspherical cavity. We find that the magnitude of the discrete wavelength-shift signal can be sufficiently enhanced by reducing the microsphere size. A reactive sensing mechanism with inverse dependence on mode volume is confirmed in experiments with virus-sized polystyrene nanoparticles. By comparing the electromagnetic theory for this reactive effect with experiments, the size and mass (≈5.2 × 10-16 g) of a bound virion are determined directly from the optimal resonance shift.

AB - We report the label-free, real-time optical detection of Influenza A virus particles. Binding of single virions is observed from discrete changes in the resonance frequency/wavelength of a whispering-gallery mode excited in a microspherical cavity. We find that the magnitude of the discrete wavelength-shift signal can be sufficiently enhanced by reducing the microsphere size. A reactive sensing mechanism with inverse dependence on mode volume is confirmed in experiments with virus-sized polystyrene nanoparticles. By comparing the electromagnetic theory for this reactive effect with experiments, the size and mass (≈5.2 × 10-16 g) of a bound virion are determined directly from the optimal resonance shift.

KW - Biosensor

KW - Influenza

KW - Optical resonance

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Single virus detection from the reactive shift of a whispering-gallery mode

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