Spatio-temporally patterned neuroectoderm tissues recapitulate early neural morphogenesis and pathogenesis

Geetika Sahni, Shu Yung Chang, Jeremy Teo, Mahmoud Pouladi, Yi Chin Toh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Current human neural differentiation model-systems can dictate neuroectoderm (NE) cell fate specification but not their morphogenesis. We report a first instance of directing the formation of a human-specific NE tissue that recapitulates architectural and cellular characteristics of early neural tube morphogenesis, including, a continuous, polarized epithelium and a distinct invagination-like folding, where the NE cells undergo apical constriction. This is accomplished by spatiotemporal patterning of the mesoendoderm that guides self-organization of the adjacent NE. Notably, evaluation of NE structural dysmorphia, which is uniquely achievable in our model, allowed for early detection of pathologies mediated by FMR1 silencing in Fragile X Syndrome.

Original languageEnglish (US)
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1423-1424
Number of pages2
ISBN (Electronic)9781510897571
StatePublished - 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: Nov 11 2018Nov 15 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume3

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period11/11/1811/15/18

Keywords

  • Morphogenesis
  • Neurodevelopmental diseases
  • Neuroectoderm
  • Stem cell micropatterning

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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