TY - JOUR
T1 - Sphingosine-1-phosphate effects on PCK isoform expression in human osteoblastic cells
AU - Lampasso, J. D.
AU - Kamer, A.
AU - Margarone, J.
AU - Dziak, R.
N1 - Funding Information:
Correspondence to:Dr Rosemary Dziak,320 Foster Hall,SUNYat Buffalo,3435 Main street,Buffalo,NY14214,USA.Tel.: (716) 829-3827; Fax: (716) 829-3942; E-mail: [email protected] This work was supported by Contract grant sponsor:NIH; Contract grant number: DE00158.
PY - 2001
Y1 - 2001
N2 - Sphingosine-1-phosphate (S1P) has been shown to participate in the proliferative process in human osteoblasts. The mitogenic effect of S1P has been postulated to involve two signaling pathways, the Gi linked protein receptor pathway and the PKC pathway. To define the possible role of PKC isoforms in osteoblastic cell proliferation, the effects of S1P on PKC isoform expression was determined. While PKC λ was minimally detected, the isoforms α, δ and ι were all found to be highly expressed by the human osteoblast. In human osteoblastic cells, S1P induced a 25% increase in the expression of PKC αand approximately a 30% increase in the expression of PKC ι. S1P did not have an effect on PKC δ expression. Pretreatment with pertussis toxin (PT) led to an inhibition of the observed S1P effects on the expression of the αand ι isoforms.
AB - Sphingosine-1-phosphate (S1P) has been shown to participate in the proliferative process in human osteoblasts. The mitogenic effect of S1P has been postulated to involve two signaling pathways, the Gi linked protein receptor pathway and the PKC pathway. To define the possible role of PKC isoforms in osteoblastic cell proliferation, the effects of S1P on PKC isoform expression was determined. While PKC λ was minimally detected, the isoforms α, δ and ι were all found to be highly expressed by the human osteoblast. In human osteoblastic cells, S1P induced a 25% increase in the expression of PKC αand approximately a 30% increase in the expression of PKC ι. S1P did not have an effect on PKC δ expression. Pretreatment with pertussis toxin (PT) led to an inhibition of the observed S1P effects on the expression of the αand ι isoforms.
UR - http://www.scopus.com/inward/record.url?scp=0035178323&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035178323&partnerID=8YFLogxK
U2 - 10.1054/plef.2001.0302
DO - 10.1054/plef.2001.0302
M3 - Article
C2 - 11728164
AN - SCOPUS:0035178323
SN - 0952-3278
VL - 65
SP - 139
EP - 146
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
IS - 3
ER -