TY - JOUR
T1 - Standardized arrabidaea chica extract shows cytoprotective effects in zoledronic acid-treated fibroblasts and osteoblasts
AU - Zago, Patricia Maria Wiziack
AU - Sousa, Ilza Maria Oliveira
AU - Servat-Medina, Leila
AU - Jorge, Michelle Pedroza
AU - Neto, Lidio Gonçalves Lima
AU - Hass, Viviane
AU - Li, Xin
AU - Ruiz, Ana Lucia Tasca Gois
AU - Saxena, Deepak
AU - Foglio, Mary Ann
N1 - Publisher Copyright:
© 2020 Wiziack Zago et al.
PY - 2020
Y1 - 2020
N2 - Introduction: Osteonecrosis of the jaw is a condition associated with intraoral ulceration and bone necrosis induced by antiresorptive medications, such as zoledronic acid, a bisphosphonate. Previous data on Arrabidaea chica (H&B.) Verlot wound healing activity prompted the study reported herein on A. chica standardized hydro alcoholic extract in vitro cytoprotective activity data on epithelial and osteoblastic cells exposed to zoledronic acid (ZA). Methods: Primary human gingival fibroblasts and murine pre-osteoblasts were treated with ZA 10 µM together with 5 or 10 µg.mL−1 A. chica extract for 24h and 48 h. At both times, cells were submitted to viability assay and caspase 3/7 activation evaluation. Statistical analysis used one-way ANOVA and p=0.05. Results: In cell viability assay, a drastic damage effect of ZA appeared after 48 h in both epithelial (55.8%) and pre-osteoblastic cells (39.7%). When treated with ZA in combination with A. chica extract, cells showed higher viability values: 74.1%–82.3% for fibroblasts and 66% for pre-osteoblasts. Furthermore, the combined treatment presented lower caspase 3/7 activation in fibroblasts and pre-osteoblasts. Conclusion: At low concentrations, A. chica extract showed promising cytoprotective effects against ZA-induced damage actions; however, further in vitro and in vivo studies are required to establish the mechanism of action.
AB - Introduction: Osteonecrosis of the jaw is a condition associated with intraoral ulceration and bone necrosis induced by antiresorptive medications, such as zoledronic acid, a bisphosphonate. Previous data on Arrabidaea chica (H&B.) Verlot wound healing activity prompted the study reported herein on A. chica standardized hydro alcoholic extract in vitro cytoprotective activity data on epithelial and osteoblastic cells exposed to zoledronic acid (ZA). Methods: Primary human gingival fibroblasts and murine pre-osteoblasts were treated with ZA 10 µM together with 5 or 10 µg.mL−1 A. chica extract for 24h and 48 h. At both times, cells were submitted to viability assay and caspase 3/7 activation evaluation. Statistical analysis used one-way ANOVA and p=0.05. Results: In cell viability assay, a drastic damage effect of ZA appeared after 48 h in both epithelial (55.8%) and pre-osteoblastic cells (39.7%). When treated with ZA in combination with A. chica extract, cells showed higher viability values: 74.1%–82.3% for fibroblasts and 66% for pre-osteoblasts. Furthermore, the combined treatment presented lower caspase 3/7 activation in fibroblasts and pre-osteoblasts. Conclusion: At low concentrations, A. chica extract showed promising cytoprotective effects against ZA-induced damage actions; however, further in vitro and in vivo studies are required to establish the mechanism of action.
KW - Arrabidaea chica
KW - Epithelial cells
KW - Fibroblasts
KW - Osteoblasts
KW - Zoledronic acid
UR - http://www.scopus.com/inward/record.url?scp=85090661839&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85090661839&partnerID=8YFLogxK
U2 - 10.2147/CCIDE.S259158
DO - 10.2147/CCIDE.S259158
M3 - Article
AN - SCOPUS:85090661839
SN - 1179-1357
VL - 12
SP - 327
EP - 333
JO - Clinical, Cosmetic and Investigational Dentistry
JF - Clinical, Cosmetic and Investigational Dentistry
ER -