Stimulation of DNA synthesis by epidermal growth factor in osteoblast-like cells

K. W. Ng, N. C. Partridge, M. Niall, T. J. Martin

Research output: Contribution to journalArticlepeer-review

Abstract

Normal and malignant osteoblast-like cells in culture have been shown to possess specific, high affinity receptors for epidermal growth factor (EGF). In this study, the mitogenic response to EGF was examined in a clonal line of a rat osteogenic sarcoma (UMR 106) and in osteoblast-rich newborn rat calvarial cells. Twenty-four hour treatment of UMR 106 cells with EGF in doses ranging from 10-12 m to 2 × 10-8 m stimulated the incorporation of [3H]thymidine and DNA synthesis in a dose-dependent manner. This short-term stimulatory effect was sustained in long-term culture with a dose-dependent increase in cell proliferation by calvarial cells. A lag period of 8 h occurred before significant stimulation of [3H]thymidine incorporation was observed. Commitment to increased incorporation of [3H]thymidine required a minimum of 6 h continuous incubation with EGF. These results establish the osteoblast as a target cell for EGF action on bone.

Original languageEnglish (US)
Pages (from-to)624-628
Number of pages5
JournalCalcified Tissue International
Volume35
Issue number1
DOIs
StatePublished - Dec 1983

Keywords

  • DNA synthesis
  • EGF
  • Osteoblasts

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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