Strain construction and screening methods for a yeast histone H3/H4 mutant library

Junbiao Dai, Jef D. Boeke

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g., bearing a reporter of interest), and the validation of individual library members is described in detail.

    Original languageEnglish (US)
    Title of host publicationChromatin Remodeling
    Subtitle of host publicationMethods and Protocols
    EditorsRandall H. Morse
    Pages1-14
    Number of pages14
    DOIs
    StatePublished - 2012

    Publication series

    NameMethods in Molecular Biology
    Volume833
    ISSN (Print)1064-3745

    Keywords

    • Chromatin remodeling
    • High-throughput mutagenesis
    • Histones modification

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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