Strategies for three-dimensional particle tracking with holographic video microscopy

Fook Chiong Cheong, Bhaskar Jyoti Krishnatreya, David G. Grier

    Research output: Contribution to journalArticlepeer-review


    The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution. In this work, we compare the performance of two complementary analysis techniques, one based on fitting to the exact Lorenz-Mie theory and the other based on phenomenological interpretation of the scattered light field reconstructed with Rayleigh-Sommerfeld back-propagation. Although Lorenz-Mie tracking provides more information and is inherently more precise, Rayleigh-Sommerfeld reconstruction is faster and more general. The two techniques agree quantitatively on colloidal spheres' in-plane positions. Their systematic differences in axial tracking can be explained in terms of the illuminated objects' light scattering properties.

    Original languageEnglish (US)
    Pages (from-to)13563-13573
    Number of pages11
    JournalOptics Express
    Issue number13
    StatePublished - Jun 21 2010

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics


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