TY - JOUR
T1 - Stress fibres are embedded in a contractile cortical network
AU - Vignaud, Timothée
AU - Copos, Calina
AU - Leterrier, Christophe
AU - Toro-Nahuelpan, Mauricio
AU - Tseng, Qingzong
AU - Mahamid, Julia
AU - Blanchoin, Laurent
AU - Mogilner, Alex
AU - Théry, Manuel
AU - Kurzawa, Laetitia
N1 - Funding Information:
We thank the live microscopy facility MuLife of IRIG/DBSCI, funded by CEA Nanobio and labex Gral, for equipment access and use. This work was supported by grants from the European Research Council (741773, AAA awarded to L.B. and 771599, ICEBERG awarded to M.T.), from Agence Nationale de la recherche ANR (ANR-14-CE11-0003-01, MaxForce awarded to L.B. and M.T.) and from the US Army Research Office (grant W911NF-17-1-0417 to A.M.). J.M. acknowledges the European Molecular Biology Laboratory (EMBL) for funding. M.T.-N. was supported by a fellowship from the EMBL Interdisciplinary (EI3POD) programme under the Marie Skłodowska-Curie Actions COFUND (664726). We finally thank A. Kawska (IlluScientia.com) for artwork in Fig.6f.
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2021/3
Y1 - 2021/3
N2 - Contractile actomyosin networks are responsible for the production of intracellular forces. There is increasing evidence that bundles of actin filaments form interconnected and interconvertible structures with the rest of the network. In this study, we explored the mechanical impact of these interconnections on the production and distribution of traction forces throughout the cell. By using a combination of hydrogel micropatterning, traction force microscopy and laser photoablation, we measured the relaxation of traction forces in response to local photoablations. Our experimental results and modelling of the mechanical response of the network revealed that bundles were fully embedded along their entire length in a continuous and contractile network of cortical filaments. Moreover, the propagation of the contraction of these bundles throughout the entire cell was dependent on this embedding. In addition, these bundles appeared to originate from the alignment and coalescence of thin and unattached cortical actin filaments from the surrounding mesh.
AB - Contractile actomyosin networks are responsible for the production of intracellular forces. There is increasing evidence that bundles of actin filaments form interconnected and interconvertible structures with the rest of the network. In this study, we explored the mechanical impact of these interconnections on the production and distribution of traction forces throughout the cell. By using a combination of hydrogel micropatterning, traction force microscopy and laser photoablation, we measured the relaxation of traction forces in response to local photoablations. Our experimental results and modelling of the mechanical response of the network revealed that bundles were fully embedded along their entire length in a continuous and contractile network of cortical filaments. Moreover, the propagation of the contraction of these bundles throughout the entire cell was dependent on this embedding. In addition, these bundles appeared to originate from the alignment and coalescence of thin and unattached cortical actin filaments from the surrounding mesh.
UR - http://www.scopus.com/inward/record.url?scp=85092726466&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85092726466&partnerID=8YFLogxK
U2 - 10.1038/s41563-020-00825-z
DO - 10.1038/s41563-020-00825-z
M3 - Article
C2 - 33077951
AN - SCOPUS:85092726466
VL - 20
SP - 410
EP - 420
JO - Nature Materials
JF - Nature Materials
SN - 1476-1122
IS - 3
ER -