TY - JOUR
T1 - Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations
AU - Qin, Wei
AU - Amin, Shady A.
AU - Lundeen, Rachel A.
AU - Heal, Katherine R.
AU - Martens-Habbena, Willm
AU - Turkarslan, Serdar
AU - Urakawa, Hidetoshi
AU - Costa, Kyle C.
AU - Hendrickson, Erik L.
AU - Wang, Tony
AU - Beck, David Ac
AU - Tiquia-Arashiro, Sonia M.
AU - Taub, Fred
AU - Holmes, Andrew D.
AU - Vajrala, Neeraja
AU - Berube, Paul M.
AU - Lowe, Todd M.
AU - Moffett, James W.
AU - Devol, Allan H.
AU - Baliga, Nitin S.
AU - Arp, Daniel J.
AU - Sayavedra-Soto, Luis A.
AU - Hackett, Murray
AU - Armbrust, E. Virginia
AU - Ingalls, Anitra E.
AU - Stahl, David A.
N1 - Publisher Copyright:
© 2018 International Society for Microbial Ecology All rights reserved 1751-7362/18.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - High representation by ammonia-oxidizing archaea (AOA) in marine systems is consistent with their high affinity for ammonia, efficient carbon fixation, and copper (Cu)-centric respiratory system. However, little is known about their response to nutrient stress. We therefore used global transcriptional and proteomic analyses to characterize the response of a model AOA, Nitrosopumilus maritimus SCM1, to ammonia starvation, Cu limitation and Cu excess. Most predicted protein-coding genes were transcribed in exponentially growing cells, and of ∼74% detected in the proteome, ∼6% were modified by N-terminal acetylation. The general response to ammonia starvation and Cu stress was downregulation of genes for energy generation and biosynthesis. Cells rapidly depleted transcripts for the A and B subunits of ammonia monooxygenase (AMO) in response to ammonia starvation, yet retained relatively high levels of transcripts for the C subunit. Thus, similar to ammonia-oxidizing bacteria, selective retention of amoC transcripts during starvation appears important for subsequent recovery, and also suggests that AMO subunit transcript ratios could be used to assess the physiological status of marine populations. Unexpectedly, cobalamin biosynthesis was upregulated in response to both ammonia starvation and Cu stress, indicating the importance of this cofactor in retaining functional integrity during times of stress.
AB - High representation by ammonia-oxidizing archaea (AOA) in marine systems is consistent with their high affinity for ammonia, efficient carbon fixation, and copper (Cu)-centric respiratory system. However, little is known about their response to nutrient stress. We therefore used global transcriptional and proteomic analyses to characterize the response of a model AOA, Nitrosopumilus maritimus SCM1, to ammonia starvation, Cu limitation and Cu excess. Most predicted protein-coding genes were transcribed in exponentially growing cells, and of ∼74% detected in the proteome, ∼6% were modified by N-terminal acetylation. The general response to ammonia starvation and Cu stress was downregulation of genes for energy generation and biosynthesis. Cells rapidly depleted transcripts for the A and B subunits of ammonia monooxygenase (AMO) in response to ammonia starvation, yet retained relatively high levels of transcripts for the C subunit. Thus, similar to ammonia-oxidizing bacteria, selective retention of amoC transcripts during starvation appears important for subsequent recovery, and also suggests that AMO subunit transcript ratios could be used to assess the physiological status of marine populations. Unexpectedly, cobalamin biosynthesis was upregulated in response to both ammonia starvation and Cu stress, indicating the importance of this cofactor in retaining functional integrity during times of stress.
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U2 - 10.1038/ismej.2017.186
DO - 10.1038/ismej.2017.186
M3 - Article
C2 - 29053148
AN - SCOPUS:85040817941
SN - 1751-7362
VL - 12
SP - 508
EP - 519
JO - ISME Journal
JF - ISME Journal
IS - 2
ER -