TY - JOUR
T1 - Structural basis of fast- And slow-severing actin-cofilactin boundaries
AU - Hocky, Glen M.
AU - Sindelar, Charles V.
AU - Cao, Wenxiang
AU - Voth, Gregory A.
AU - De La Cruz, Enrique M.
N1 - Funding Information:
This work was supported in part by DOD ARO MURI W911NF1410403, on which G. A. V. and E. M. D. L. C. are coinvestigators, and by NIH R35-GM136656 (awarded to E. M. D. L. C.). Support for G.M. H. was provided by NIH R35-GM138312. C. V. S. was supported by NIH R01-GM-110530.
Publisher Copyright:
© 2021 The Authors.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Members of the ADF/cofilin family of regulatory proteins bind actin filaments cooperatively, locally change actin subunit conformation and orientation, and sever filaments at "boundaries"between bare and cofilin-occupied segments. A cluster of bound cofilin introduces two distinct classes of boundaries due to the intrinsic polarity of actin filaments, one at the "pointed"end side and the other at the "barbed"end-side of the cluster; severing occurs more readily at the pointed end side of the cluster ("fast-severing"boundary) than the barbed end side ("slow-severing"boundary). A recent electron-cryomicroscopy (cryo-EM) model of the slow-severing boundary revealed structural "defects"at the interface that potentially contribute to severing. However, the structure of the fast-severing boundary remains uncertain. Here, we use extensive molecular dynamics simulations to produce atomic resolution models of both severing boundaries. Our equilibrated simulation model of the slow-severing boundary is consistent with the cryo-EM structural model. Simulations indicate that actin subunits at both boundaries adopt structures intermediate between those of bare and cofilin-bound actin subunits. These "intermediate"states have compromised intersubunit contacts, but those at the slow-severing boundary are stabilized by cofilin bridging interactions, accounting for its lower fragmentation probability. Simulations where cofilin proteins are removed from cofilactin filaments favor a mechanism in which a cluster of two contiguously bound cofilins is needed to fully stabilize the cofilactin conformation, promote cooperative binding interactions, and accelerate filament severing. Together, these studies provide a molecular-scale foundation for developing coarse-grained and theoretical descriptions of cofilin-mediated actin filament severing.
AB - Members of the ADF/cofilin family of regulatory proteins bind actin filaments cooperatively, locally change actin subunit conformation and orientation, and sever filaments at "boundaries"between bare and cofilin-occupied segments. A cluster of bound cofilin introduces two distinct classes of boundaries due to the intrinsic polarity of actin filaments, one at the "pointed"end side and the other at the "barbed"end-side of the cluster; severing occurs more readily at the pointed end side of the cluster ("fast-severing"boundary) than the barbed end side ("slow-severing"boundary). A recent electron-cryomicroscopy (cryo-EM) model of the slow-severing boundary revealed structural "defects"at the interface that potentially contribute to severing. However, the structure of the fast-severing boundary remains uncertain. Here, we use extensive molecular dynamics simulations to produce atomic resolution models of both severing boundaries. Our equilibrated simulation model of the slow-severing boundary is consistent with the cryo-EM structural model. Simulations indicate that actin subunits at both boundaries adopt structures intermediate between those of bare and cofilin-bound actin subunits. These "intermediate"states have compromised intersubunit contacts, but those at the slow-severing boundary are stabilized by cofilin bridging interactions, accounting for its lower fragmentation probability. Simulations where cofilin proteins are removed from cofilactin filaments favor a mechanism in which a cluster of two contiguously bound cofilins is needed to fully stabilize the cofilactin conformation, promote cooperative binding interactions, and accelerate filament severing. Together, these studies provide a molecular-scale foundation for developing coarse-grained and theoretical descriptions of cofilin-mediated actin filament severing.
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U2 - 10.1016/j.jbc.2021.100337
DO - 10.1016/j.jbc.2021.100337
M3 - Article
C2 - 33508320
AN - SCOPUS:85100893617
VL - 296
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
M1 - 100337
ER -