TY - JOUR
T1 - Structural characterization of synthetic model peptides of the DNA‐binding cI434 repressor by electrospray ionization and fast atom bombardment mass spectrometry
AU - Percipalle, Piergiorgio
AU - Saletti, Rosaria
AU - Pongor, Sandor
AU - Foti, Salvatore
AU - Tossi, Alessandro
AU - Fisichella, Salvatore
PY - 1994/12
Y1 - 1994/12
N2 - The structural characterization of two synthetic model peptides of the cI434 repressor is described. Unequivocal determination of the structure was achieved by means of electrospray ionization mass spectrometry of the intact peptides and by fast atom bombardment mass spectrometric identification of complementary peptide fragments obtained by tryptic and chymotrypic digestion and partial separation by reversed‐phase high‐performance liquid chromatography. The results show the potential of this approach for characterizing synthetic peptides of relatively high molecular weight.
AB - The structural characterization of two synthetic model peptides of the cI434 repressor is described. Unequivocal determination of the structure was achieved by means of electrospray ionization mass spectrometry of the intact peptides and by fast atom bombardment mass spectrometric identification of complementary peptide fragments obtained by tryptic and chymotrypic digestion and partial separation by reversed‐phase high‐performance liquid chromatography. The results show the potential of this approach for characterizing synthetic peptides of relatively high molecular weight.
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U2 - 10.1002/bms.1200231203
DO - 10.1002/bms.1200231203
M3 - Article
C2 - 7841207
AN - SCOPUS:0028170481
SN - 1052-9306
VL - 23
SP - 727
EP - 733
JO - Biological Mass Spectrometry
JF - Biological Mass Spectrometry
IS - 12
ER -