Structural characterization of synthetic model peptides of the DNA‐binding cI434 repressor by electrospray ionization and fast atom bombardment mass spectrometry

Piergiorgio Percipalle, Rosaria Saletti, Sandor Pongor, Salvatore Foti, Alessandro Tossi, Salvatore Fisichella

Research output: Contribution to journalArticle

Abstract

The structural characterization of two synthetic model peptides of the cI434 repressor is described. Unequivocal determination of the structure was achieved by means of electrospray ionization mass spectrometry of the intact peptides and by fast atom bombardment mass spectrometric identification of complementary peptide fragments obtained by tryptic and chymotrypic digestion and partial separation by reversed‐phase high‐performance liquid chromatography. The results show the potential of this approach for characterizing synthetic peptides of relatively high molecular weight.

Original languageEnglish (US)
Pages (from-to)727-733
Number of pages7
JournalBiological Mass Spectrometry
Volume23
Issue number12
DOIs
StatePublished - Dec 1994

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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