Structure of Arp2/3 complex at a branched actin filament junction resolved by single-particle cryo-electron microscopy

Bojian Ding, Heidy Y. Narvaez-Ortiz, Yuvraj Singh, Glen M. Hocky, Saikat Chowdhury, Brad J. Nolen

Research output: Contribution to journalArticlepeer-review

Abstract

SignificanceActin filament nucleation by Arp2/3 complex must be triggered by activators like WASP family proteins. Understanding how WASP proteins activate Arp2/3 complex has been a major challenge due to a lack of high-resolution structures of the complex in an activated state. We determined a high-resolution (∼3.9 Å) structure of the WASP-activated Arp2/3 complex at a branch junction and used biochemical, cell biological, and molecular dynamic simulations to understand the mechanism of WASP-mediated activation. This work shows in detail the contacts between the fully activated Arp2/3 complex, the nucleated daughter actin filament, and the mother actin filament and provides important insights into how conformational rearrangements in the Arp2/3 complex are stimulated during activation.

Original languageEnglish (US)
Pages (from-to)e2202723119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number22
DOIs
StatePublished - May 31 2022

Keywords

  • actin
  • Arp2/3
  • cryo-electron microscopy
  • cytoskeleton
  • nucleation

ASJC Scopus subject areas

  • General

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