Structure of P401 (mast cell degranulating peptide) in solution

N. Vasant Kumar, D. E. Wemmer, N. R. Kallenbach

Research output: Contribution to journalArticlepeer-review


P401 (also known as mast cell degranulating protein, MCD) is a minor component of honeybee venom. Its primary structure is related to that of apamin. We have studied the structure of P401 in solution by high-resolution two-dimensional 1H-NMR spectroscopy. Almost all the backbone proton resonances have been assigned by sequential assignment strategy. Analysis of NOEs shows that P401 has a conformation very similar to that of apamin. N-terminal residues Ile-1-Cys-5 are in an extended conformation and residues His-13-Asn-22 on the C-terminus are in an α-helical structure. These two secondary structural elements are connected by two tight turns.

Original languageEnglish (US)
Pages (from-to)113-119
Number of pages7
JournalBiophysical Chemistry
Issue number1-2
StatePublished - Aug 1988


  • Mast cell degranulating protein
  • P401
  • Toxin structure
  • two-dimensional NMR

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry


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