Abstract
P401 (also known as mast cell degranulating protein, MCD) is a minor component of honeybee venom. Its primary structure is related to that of apamin. We have studied the structure of P401 in solution by high-resolution two-dimensional 1H-NMR spectroscopy. Almost all the backbone proton resonances have been assigned by sequential assignment strategy. Analysis of NOEs shows that P401 has a conformation very similar to that of apamin. N-terminal residues Ile-1-Cys-5 are in an extended conformation and residues His-13-Asn-22 on the C-terminus are in an α-helical structure. These two secondary structural elements are connected by two tight turns.
Original language | English (US) |
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Pages (from-to) | 113-119 |
Number of pages | 7 |
Journal | Biophysical Chemistry |
Volume | 31 |
Issue number | 1-2 |
DOIs | |
State | Published - Aug 1988 |
Keywords
- Mast cell degranulating protein
- P401
- Toxin structure
- two-dimensional NMR
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Organic Chemistry