Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP

Amédée Des Georges, Yaser Hashem, Anett Unbehaun, Robert A. Grassucci, Derek Taylor, Christopher U.T. Hellen, Tatyana V. Pestova, Joachim Frank

Research output: Contribution to journalArticlepeer-review


Eukaryotic translation termination results from the complex functional interplay between two release factors, eRF1 and eRF3, in which GTP hydrolysis by eRF3 couples codon recognition with peptidyl-tRNA hydrolysis by eRF1. Here, we present a cryo-electron microscopy structure of pre-termination complexes associated with eRF1•eRF3•GDPNP at 9.7 -Å resolution, which corresponds to the initial pre-GTP hydrolysis stage of factor attachment and stop codon recognition. It reveals the ribosomal positions of eRFs and provides insights into the mechanisms of stop codon recognition and triggering of eRF3's GTPase activity.

Original languageEnglish (US)
Pages (from-to)3409-3418
Number of pages10
JournalNucleic acids research
Issue number5
StatePublished - Mar 1 2014

ASJC Scopus subject areas

  • Genetics


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