TY - JOUR
T1 - Substance P induces interleukin-8 secretion from human dental pulp cells
AU - Patel, Tushar
AU - Park, Sang Hyuk
AU - Lin, Louis M.
AU - Chiappelli, Francesco
AU - Huang, George T.J.
N1 - Funding Information:
Supported in part by an Endodontic Research Grant from American Association of Endodontists Foundation and a UCLA School of Dentistry Research Opportunity Grant.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Objective. The role of neuropeptides in recruiting leukocytes in dental pulps is not known; therefore, we investigated whether interleukin-8 (IL-8) secretion from human pulp cells is increased after stimulation with substance P (SP) or calcitonin gene-related peptide (CGRP). Methods. Primary pulp cells exhibiting a fibroblast-like phenotype and an endothelial cell line were stimulated with various doses of SP or CGRP, and IL-8 secretion was measured by enzyme-linked immunosorbent assays. Results. IL-8 secretion from pulp cells increased significantly at 10-8 to 10-4 mol/L of SP stimulation (5- to 13.8-fold; P < .05); however, no significant IL-8 increase with CGRP (up to 10-4 mol/L) stimulation was observed, nor was there synergistic induction of IL-8 with SP plus CGRP. The IL-8 increase reached its maximum at 8 hours after SP stimulation of the pulp cells. SP at a concentration of 10-4 mol/L created minimal IL-8 induction in the endothelial cells and no synergistic induction by SP and CGRP. Conclusions. Pulp cells may up-regulate IL-8 secretion by SP stimulation, which suggests that SP released in dental pulp may play a role in the influx of leukocytes, attracted by IL-8, into the pulp tissue.
AB - Objective. The role of neuropeptides in recruiting leukocytes in dental pulps is not known; therefore, we investigated whether interleukin-8 (IL-8) secretion from human pulp cells is increased after stimulation with substance P (SP) or calcitonin gene-related peptide (CGRP). Methods. Primary pulp cells exhibiting a fibroblast-like phenotype and an endothelial cell line were stimulated with various doses of SP or CGRP, and IL-8 secretion was measured by enzyme-linked immunosorbent assays. Results. IL-8 secretion from pulp cells increased significantly at 10-8 to 10-4 mol/L of SP stimulation (5- to 13.8-fold; P < .05); however, no significant IL-8 increase with CGRP (up to 10-4 mol/L) stimulation was observed, nor was there synergistic induction of IL-8 with SP plus CGRP. The IL-8 increase reached its maximum at 8 hours after SP stimulation of the pulp cells. SP at a concentration of 10-4 mol/L created minimal IL-8 induction in the endothelial cells and no synergistic induction by SP and CGRP. Conclusions. Pulp cells may up-regulate IL-8 secretion by SP stimulation, which suggests that SP released in dental pulp may play a role in the influx of leukocytes, attracted by IL-8, into the pulp tissue.
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U2 - 10.1016/S1079-2104(03)00036-2
DO - 10.1016/S1079-2104(03)00036-2
M3 - Article
C2 - 14561975
AN - SCOPUS:0141887422
SN - 1079-2104
VL - 96
SP - 478
EP - 485
JO - Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
JF - Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
IS - 4
ER -