Substance P induces interleukin-8 secretion from human dental pulp cells

Tushar Patel, Sang Hyuk Park, Louis M. Lin, Francesco Chiappelli, George T.J. Huang

Research output: Contribution to journalArticlepeer-review


Objective. The role of neuropeptides in recruiting leukocytes in dental pulps is not known; therefore, we investigated whether interleukin-8 (IL-8) secretion from human pulp cells is increased after stimulation with substance P (SP) or calcitonin gene-related peptide (CGRP). Methods. Primary pulp cells exhibiting a fibroblast-like phenotype and an endothelial cell line were stimulated with various doses of SP or CGRP, and IL-8 secretion was measured by enzyme-linked immunosorbent assays. Results. IL-8 secretion from pulp cells increased significantly at 10-8 to 10-4 mol/L of SP stimulation (5- to 13.8-fold; P < .05); however, no significant IL-8 increase with CGRP (up to 10-4 mol/L) stimulation was observed, nor was there synergistic induction of IL-8 with SP plus CGRP. The IL-8 increase reached its maximum at 8 hours after SP stimulation of the pulp cells. SP at a concentration of 10-4 mol/L created minimal IL-8 induction in the endothelial cells and no synergistic induction by SP and CGRP. Conclusions. Pulp cells may up-regulate IL-8 secretion by SP stimulation, which suggests that SP released in dental pulp may play a role in the influx of leukocytes, attracted by IL-8, into the pulp tissue.

Original languageEnglish (US)
Pages (from-to)478-485
Number of pages8
JournalOral surgery, oral medicine, oral pathology, oral radiology, and endodontics
Issue number4
StatePublished - Oct 1 2003

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • General Dentistry


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