Subtype-selective electroporation of cortical interneurons

Natalia V. De Marco Garcia, Gord Fishel

Research output: Contribution to journalArticlepeer-review

Abstract

The study of central nervous system (CNS) maturation relies on genetic targeting of neuronal populations. However, the task of restricting the expression of genes of interest to specific neuronal subtypes has proven remarkably challenging due to the relative scarcity of specific promoter elements. GABAergic interneurons constitute a neuronal population with extensive genetic and morphological diversity. Indeed, more than 11 different subtypes of GABAergic interneurons have been characterized in the mouse cortex1. Here we present an adapted protocol for selective targeting of GABAergic populations. We achieved subtype selective targeting of GABAergic interneurons by using the enhancer element of the homeobox transcription factors Dlx5 and Dlx6, homologues of the Drosophila distal-less (Dll) gene2,3, to drive the expression of specific genes through in utero electroporation.

Original languageEnglish (US)
Article numbere51518
JournalJournal of Visualized Experiments
Issue number90
DOIs
StatePublished - Aug 18 2014

Keywords

  • Cortex
  • Development
  • Electroporation
  • Interneurons
  • Issue 90
  • Morphology
  • Mouse
  • Neuroscience

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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