1 H-decoupling and Isotopic Labeling for the Measurement of the Longitudinal Relaxation Time of Hyperpolarized 13 C-Methylenes in Choline Analogs

Sivaranjan Uppala, Ayelet Gamliel, Talia Harris, Jacob Sosna, J. Moshe Gomori, Alexej Jerschow, Rachel Katz-Brull

Research output: Contribution to journalArticle

Abstract

The T 1 of a hyperpolarized site in solution is a key parameter that determines the time-window in which its NMR signals are observable. For 13 C sites adjacent to protons, 1 H-decoupling has been shown to increase the hyperpolarized signal resolution and SNR. Additionally, polarization transfer to protons has shown utility in increasing the sensitivity of detection. However, 1 H-decoupling could lead to a change in the decay rate of a hyperpolarized 13 C site. Here we tested this possible effect in a case where the protons are directly bound to an sp 3 hyperpolarized 13 C site (using [1,2- 13 C 2 ]choline) and 1 H-decoupling was applied continuously throughout the hyperpolarized decay measurement. We found that 1 H-decoupling did not lead to any significant changes in the 13 C polarization decay time but did result in the expected collapse of J-coupling and produced sharper signals. This result suggested that 1 H-decoupling did not affect the decay rate of hyperpolarized sp 3 13 C sites. The deuterium-substitution approach (using [1,1,2,2-D 4 ,1- 13 C]choline) showed a dramatic prolongation of T 1 . Upper bounds on the T 1 of all investigated sites were calculated.

Original languageEnglish (US)
JournalIsrael Journal of Chemistry
DOIs
StatePublished - Jan 1 2019

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Choline
Relaxation time
Labeling
Protons
Polarization
Deuterium
Substitution reactions
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

1 H-decoupling and Isotopic Labeling for the Measurement of the Longitudinal Relaxation Time of Hyperpolarized 13 C-Methylenes in Choline Analogs . / Uppala, Sivaranjan; Gamliel, Ayelet; Harris, Talia; Sosna, Jacob; Gomori, J. Moshe; Jerschow, Alexej; Katz-Brull, Rachel.

In: Israel Journal of Chemistry, 01.01.2019.

Research output: Contribution to journalArticle

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