1H-decoupling and Isotopic Labeling for the Measurement of the Longitudinal Relaxation Time of Hyperpolarized 13C-Methylenes in Choline Analogs

Sivaranjan Uppala, Ayelet Gamliel, Talia Harris, Jacob Sosna, J. Moshe Gomori, Alexej Jerschow, Rachel Katz-Brull

Research output: Contribution to journalArticle

Abstract

The T1 of a hyperpolarized site in solution is a key parameter that determines the time-window in which its NMR signals are observable. For 13C sites adjacent to protons, 1H-decoupling has been shown to increase the hyperpolarized signal resolution and SNR. Additionally, polarization transfer to protons has shown utility in increasing the sensitivity of detection. However, 1H-decoupling could lead to a change in the decay rate of a hyperpolarized 13C site. Here we tested this possible effect in a case where the protons are directly bound to an sp3 hyperpolarized 13C site (using [1,2-13C2]choline) and 1H-decoupling was applied continuously throughout the hyperpolarized decay measurement. We found that 1H-decoupling did not lead to any significant changes in the 13C polarization decay time but did result in the expected collapse of J-coupling and produced sharper signals. This result suggested that 1H-decoupling did not affect the decay rate of hyperpolarized sp3 13C sites. The deuterium-substitution approach (using [1,1,2,2-D4,1-13C]choline) showed a dramatic prolongation of T1. Upper bounds on the T1 of all investigated sites were calculated.

Original languageEnglish (US)
Pages (from-to)1014-1019
Number of pages6
JournalIsrael Journal of Chemistry
Volume59
Issue number11-12
DOIs
StatePublished - Nov 1 2019

Keywords

  • Carbon-13
  • T1
  • dynamic nuclear polarization
  • polarization transfer
  • proton decoupling

ASJC Scopus subject areas

  • Chemistry(all)

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