31P NMR of DNA in eukaryotic chromosomal complexes

J. A. DiVerdi, S. J. Opella, R. I. Ma, N. R. Kallenbach, N. C. Seeman

Research output: Contribution to journalArticle

Abstract

DNA complexed with histones in soluble chromatin and with protamines in the heads of sperm has been studied by 31P NMR spectroscopy. Because of the large size of these nucleoprotein structures, methods of high resolution solid state NMR were employed. Proton decoupled 31P NMR spectra of these complexes in solution yield anisotropic chemical shift powder patterns, which indicate that the DNA is substantially immobilized by interactions with the proteins. Rapid rotation of these samples at the magic angle gives single line spectra with an isotropic chemical shift indistinguishable from DNA in the absence of proteins or that in mononucleosome core particles; this argues that packaging of the DNA by the proteins does not introduce major distortions in a predominant fraction of the phosphodiester linkages present.

Original languageEnglish (US)
Pages (from-to)885-890
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume102
Issue number3
DOIs
StatePublished - Oct 15 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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