[3H]5‐Hydroxytryptamine Binding to Brain Astroglial Cells: Differences Between Intact and Homogenized Preparations and Mature and Immature Cultures

Patricia M. Whitaker‐Azmitia; Efrain C. Azmitia

doi:10.1111/j.1471-4159.1986.tb00636.x

[³H]5‐Hydroxytryptamine Binding to Brain Astroglial Cells: Differences Between Intact and Homogenized Preparations and Mature and Immature Cultures

Patricia M. Whitaker‐Azmitia, Efrain C. Azmitia

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: [³H]5‐hydroxytryptamine ([³H]serotonin) binds with high affinity (K_D 2–12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C₆ glioma line is decreased significantly (B_max= 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (B_max= 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.

Original language	English (US)
Pages (from-to)	1186-1189
Number of pages	4
Journal	Journal of Neurochemistry
Volume	46
Issue number	4
DOIs	https://doi.org/10.1111/j.1471-4159.1986.tb00636.x
State	Published - Apr 1986

Keywords

Astroglial cells
Neuronal development
Serotonin
Serotonin receptor

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

Access to Document

10.1111/j.1471-4159.1986.tb00636.x

Cite this

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title = "[3H]5‐Hydroxytryptamine Binding to Brain Astroglial Cells: Differences Between Intact and Homogenized Preparations and Mature and Immature Cultures",

abstract = "Abstract: [3H]5‐hydroxytryptamine ([3H]serotonin) binds with high affinity (KD 2–12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C6 glioma line is decreased significantly (Bmax= 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (Bmax= 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.",

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AB - Abstract: [3H]5‐hydroxytryptamine ([3H]serotonin) binds with high affinity (KD 2–12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C6 glioma line is decreased significantly (Bmax= 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (Bmax= 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.

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