[3H]5‐Hydroxytryptamine Binding to Brain Astroglial Cells: Differences Between Intact and Homogenized Preparations and Mature and Immature Cultures

Patricia M. Whitaker‐Azmitia, Efrain C. Azmitia

Research output: Contribution to journalArticle

Abstract

Abstract: [3H]5‐hydroxytryptamine ([3H]serotonin) binds with high affinity (KD 2–12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C6 glioma line is decreased significantly (Bmax= 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (Bmax= 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.

Original languageEnglish (US)
Pages (from-to)1186-1189
Number of pages4
JournalJournal of Neurochemistry
Volume46
Issue number4
DOIs
StatePublished - Apr 1986

Keywords

  • Astroglial cells
  • Neuronal development
  • Serotonin
  • Serotonin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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