Supplementing a low-protein diet with dibasic amino acids increases urinary calcium excretion in young women

Jessica D. Bihuniak, Rebecca R. Sullivan, Christine A. Simpson, Donna M. Caseria, Tania B. Huedo-Medina, Kimberly O. O'Brien, Jane E. Kerstetter, Karl L. Insogna

Research output: Contribution to journalArticlepeer-review


Increasing dietary protein within a physiologic range stimulates intestinal calcium absorption, but it is not known if specific amino acids or dietary protein as a whole are responsible for this effect. Therefore, we selectively supplemented a lowprotein (0.7 g/kg) diet with either the calcium-sensing receptor-activating amino acids (CaSR-AAAs) L-tryptophan, L-phenylalanine, and L-histidine, or the dibasic amino acids (DAAs) L-arginine and L-lysine, to achieve intakes comparable to the content of a high-protein diet (2.1 g/kg) and measured intestinal calcium absorption. Fourteen young women took part in a placebo-controlled, double-blind, crossover feeding trial in which each participant ingested a 6-d low-protein diet supplemented with CaSR-AAAs, DAAs, or methylcellulose capsules (control) after an 11-d adjustment period. All participants ingested all 3 diets in random order. Intestinal calcium absorption was measured between days 5 and 6 using dual-stable calcium isotopes (42Ca, 43Ca, and 44Ca). There was no difference in calcium absorption between the diet supplemented with CaSR-AAAs (22.9 ± 2.0%) and the control diet (22.3 ± 1.4%) (P = 0.64). However, calcium absorption tended to be greater during the DAA supplementation period (25.2 ± 1.4%) compared with the control diet period (22.3 ± 1.4%) (P < 0.10). Larger and longer clinical trials are needed to clarify the possible benefit of arginine and lysine on calcium absorption.

Original languageEnglish (US)
Pages (from-to)282-288
Number of pages7
JournalJournal of Nutrition
Issue number3
StatePublished - Mar 1 2014

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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