Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa

Ann V. Isaac, Sandhya Kumari, Ramya Nair, Deepak Raj Urs, Sujith Raj Salian, Guruprasad Kalthur, Satish Kumar Adiga, Jyothsna Manikkath, Srinivas Mutalik, Divya Sachdev, Renu Pasricha

Research output: Contribution to journalArticlepeer-review


The sperm DNA integrity post cryopreservation of human semen samples is one of the serious concerns in human infertility treatment. In the present study, the beneficial effects of zinc oxide nanoparticles in preserving the functional ability of spermatozoa was explored. Ejaculates of normozoospermic men cryopreserved along with Zinc oxide nanoparticles (ZnONPs) exhibited non-significantly higher percentage of total and progressive motility in frozen-thawed samples compared to control. The sperm chromatin damage and malondialdehyde (MDA) level was significantly lower in ZnONPs group (P < 0.01 and P < 0.05 respectively) and the spermatozoa's ability to undergo acrosome reaction was also unaltered. Fluorescence microscopy and High resolution transmission electron microscopy analysis demonstrated that the ZnONPs do not penetrate the membrane of spermatozoa but stay around the spermatozoa. In conclusion, the presence of ZnONPs during cryopreservation appears to be beneficial to the spermatozoa as they withstand freeze-thaw process competently better than control, without any adverse effect shown.

Original languageEnglish (US)
Pages (from-to)656-662
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3-4
StatePublished - Dec 16 2017


  • DNA integrity
  • Lipid peroxidation
  • Semen cryopreservation
  • Transmission electron microscopy
  • Zinc oxide nanoparticles

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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