Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa

Ann V. Isaac; Sandhya Kumari; Ramya Nair; Deepak Raj Urs; Sujith Raj Salian; Guruprasad Kalthur; Satish Kumar Adiga; Jyothsna Manikkath; Srinivas Mutalik; Divya Sachdev; Renu Pasricha

doi:10.1016/j.bbrc.2017.10.112

Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa

Ann V. Isaac, Sandhya Kumari, Ramya Nair, Deepak Raj Urs, Sujith Raj Salian, Guruprasad Kalthur, Satish Kumar Adiga, Jyothsna Manikkath, Srinivas Mutalik, Divya Sachdev, Renu Pasricha

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Abstract

The sperm DNA integrity post cryopreservation of human semen samples is one of the serious concerns in human infertility treatment. In the present study, the beneficial effects of zinc oxide nanoparticles in preserving the functional ability of spermatozoa was explored. Ejaculates of normozoospermic men cryopreserved along with Zinc oxide nanoparticles (ZnONPs) exhibited non-significantly higher percentage of total and progressive motility in frozen-thawed samples compared to control. The sperm chromatin damage and malondialdehyde (MDA) level was significantly lower in ZnONPs group (P < 0.01 and P < 0.05 respectively) and the spermatozoa's ability to undergo acrosome reaction was also unaltered. Fluorescence microscopy and High resolution transmission electron microscopy analysis demonstrated that the ZnONPs do not penetrate the membrane of spermatozoa but stay around the spermatozoa. In conclusion, the presence of ZnONPs during cryopreservation appears to be beneficial to the spermatozoa as they withstand freeze-thaw process competently better than control, without any adverse effect shown.

Original language	English (US)
Pages (from-to)	656-662
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	494
Issue number	3-4
DOIs	https://doi.org/10.1016/j.bbrc.2017.10.112
State	Published - Dec 16 2017

Keywords

DNA integrity
Lipid peroxidation
Semen cryopreservation
Transmission electron microscopy
Zinc oxide nanoparticles

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Isaac, A. V., Kumari, S., Nair, R., Urs, D. R., Salian, S. R., Kalthur, G., Adiga, S. K., Manikkath, J., Mutalik, S., Sachdev, D., & Pasricha, R. (2017). Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa. Biochemical and Biophysical Research Communications, 494(3-4), 656-662. https://doi.org/10.1016/j.bbrc.2017.10.112

Isaac, AV, Kumari, S, Nair, R, Urs, DR, Salian, SR, Kalthur, G, Adiga, SK, Manikkath, J, Mutalik, S, Sachdev, D & Pasricha, R 2017, 'Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa', Biochemical and Biophysical Research Communications, vol. 494, no. 3-4, pp. 656-662. https://doi.org/10.1016/j.bbrc.2017.10.112

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abstract = "The sperm DNA integrity post cryopreservation of human semen samples is one of the serious concerns in human infertility treatment. In the present study, the beneficial effects of zinc oxide nanoparticles in preserving the functional ability of spermatozoa was explored. Ejaculates of normozoospermic men cryopreserved along with Zinc oxide nanoparticles (ZnONPs) exhibited non-significantly higher percentage of total and progressive motility in frozen-thawed samples compared to control. The sperm chromatin damage and malondialdehyde (MDA) level was significantly lower in ZnONPs group (P < 0.01 and P < 0.05 respectively) and the spermatozoa's ability to undergo acrosome reaction was also unaltered. Fluorescence microscopy and High resolution transmission electron microscopy analysis demonstrated that the ZnONPs do not penetrate the membrane of spermatozoa but stay around the spermatozoa. In conclusion, the presence of ZnONPs during cryopreservation appears to be beneficial to the spermatozoa as they withstand freeze-thaw process competently better than control, without any adverse effect shown.",

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AU - Isaac, Ann V.

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AU - Urs, Deepak Raj

AU - Salian, Sujith Raj

AU - Kalthur, Guruprasad

AU - Adiga, Satish Kumar

AU - Manikkath, Jyothsna

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AU - Sachdev, Divya

AU - Pasricha, Renu

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AB - The sperm DNA integrity post cryopreservation of human semen samples is one of the serious concerns in human infertility treatment. In the present study, the beneficial effects of zinc oxide nanoparticles in preserving the functional ability of spermatozoa was explored. Ejaculates of normozoospermic men cryopreserved along with Zinc oxide nanoparticles (ZnONPs) exhibited non-significantly higher percentage of total and progressive motility in frozen-thawed samples compared to control. The sperm chromatin damage and malondialdehyde (MDA) level was significantly lower in ZnONPs group (P < 0.01 and P < 0.05 respectively) and the spermatozoa's ability to undergo acrosome reaction was also unaltered. Fluorescence microscopy and High resolution transmission electron microscopy analysis demonstrated that the ZnONPs do not penetrate the membrane of spermatozoa but stay around the spermatozoa. In conclusion, the presence of ZnONPs during cryopreservation appears to be beneficial to the spermatozoa as they withstand freeze-thaw process competently better than control, without any adverse effect shown.

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Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa

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Keywords

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