Sustained effects of developmental exposure to inorganic arsenic on hepatic gsto2 expression and mating success in zebrafish

Abigail Ama Koomson, Patrice Delaney, Nouf Khan, Kirsten C. Sadler

Research output: Contribution to journalArticlepeer-review


The impacts of exposure to the pervasive environmental toxicant, inorganic arsenic (iAs), on human and fish health are well characterized and several lines of evidence suggest that some impacts can manifest years after exposure cessation. Using a developmental exposure protocol whereby zebrafish embryos were exposed to 0.5 and 1.5 mM iAs from 4–120 hours post fertilization (hpf) and then removed, we investigated the sustained effects of iAs on gene expression in the liver, survival, reproductive success, and susceptibility to iAs toxicity in the subsequent generation. Persistent exposure to iAs during development had substantial effects on the hepatic transcriptome, with 23% of all expressed genes significantly changed following developmental exposure. The gsto2 gene is involved in iAs metabolism and this gene was significantly downregulated in female livers 9 months after iAs was removed. Developmental exposure to 1.5 mM iAs, but not 0.5 mM, decreased survival by over 50% at 3 months of age. Adults that were developmentally exposed to 0.5 mM iAs had reduced mating success, but their offspring had no differences in observable aspects of development or their susceptibility to iAs toxicity. This demonstrates that developmental exposure of zebrafish to iAs reduces long-term survival, reproductive success and causes sustained changes to gsto2 expression in the liver.

Original languageEnglish (US)
Article numberbio060094
JournalBiology Open
Issue number3
StatePublished - Mar 2024


  • Arsenic
  • Developmental origins of health
  • Liver
  • Zebrafish
  • disease

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


Dive into the research topics of 'Sustained effects of developmental exposure to inorganic arsenic on hepatic gsto2 expression and mating success in zebrafish'. Together they form a unique fingerprint.

Cite this