@article{25445998362f465db26af868a10bd826,
title = "SWR1-Independent Association of H2A.Z to the LINC Complex Promotes Meiotic Chromosome Motion",
abstract = "The H2A.Z histone variant is deposited into the chromatin by the SWR1 complex, affecting multiple aspects of meiosis. We describe here a SWR1-independent localization of H2A.Z at meiotic telomeres and the centrosome. We demonstrate that H2A.Z colocalizes and interacts with Mps3, the SUN component of the linker of nucleoskeleton, and cytoskeleton (LINC) complex that spans the nuclear envelope and links meiotic telomeres to the cytoskeleton, promoting meiotic chromosome movement. H2A.Z also interacts with the meiosis-specific Ndj1 protein that anchors telomeres to the nuclear periphery via Mps3. Telomeric localization of H2A.Z depends on Ndj1 and the N-terminal domain of Mps3. Although telomeric attachment to the nuclear envelope is maintained in the absence of H2A.Z, the distribution of Mps3 is altered. The velocity of chromosome movement during the meiotic prophase is reduced in the htz1Δ mutant lacking H2A.Z, but it is unaffected in swr1Δ cells. We reveal that H2A.Z is an additional LINC-associated factor that contributes to promote telomere-driven chromosome motion critical for error-free gametogenesis.",
keywords = "H2A.Z, LINC complex, Mps3, Ndj1, chromosome movement, meiosis, yeast",
author = "Sara Gonz{\'a}lez-Arranz and Gardner, {Jennifer M.} and Zulin Yu and Patel, {Neem J.} and Jonna Heldrich and Beatriz Santos and Carballo, {Jes{\'u}s A.} and Jaspersen, {Sue L.} and Andreas Hochwagen and San-Segundo, {Pedro A.}",
note = "Funding Information: We thank Andr?s Clemente and F?lix Prado for useful comments and discussion, Cristina Mart?n, Andr?s Clemente, C?sar Roncero, Raimundo Freire, and Shirleen Roeder for reagents, and Giovanni Cardone for the image alignment script. Funding. This work was supported by grant BFU2015-65417-R from the Ministry of Economy and Competitiveness (MINECO) of Spain to PS-S, grant RTI2018-099055-B-I00 from the Ministry of Science, Innovation and Universities (MCIU/AEI/FEDER, UE) of Spain to PS-S and JC, the Stowers Institute for Medical Research to SJ, grant R01GM121443 from the National Institute of General Medical Sciences of the National Institutes of Health to SJ, and NIH grants GM111715 and GM123035 to AH. SG-A was partially supported by the Unidad de Excelencia de Biolog?a Funcional y Gen?mica Ref. 2019/X002/05 from the University of Salamanca. The IBFG was supported in part by an institutional grant from the Junta de Castilla y Le?n, Ref. CLU-2017-03 co-funded by the P.O. FEDER de Castilla y Le?n 14-20. This manuscript has been released as a preprint at bioRxiv (Gonz?lez-Arranz et al., 2020). Open Access publication fee is partially supported by the CSIC. Funding Information: This work was supported by grant BFU2015-65417-R from the Ministry of Economy and Competitiveness (MINECO) of Spain to PS-S, grant RTI2018-099055-B-I00 from the Ministry of Science, Innovation and Universities (MCIU/AEI/FEDER, UE) of Spain to PS-S and JC, the Stowers Institute for Medical Research to SJ, grant R01GM121443 from the National Institute of General Medical Sciences of the National Institutes of Health to SJ, and NIH grants GM111715 and GM123035 to AH. SG-A was partially supported by the Unidad de Excelencia de Biolog{\'i}a Funcional y Gen{\'o}mica Ref. 2019/X002/05 from the University of Salamanca. The IBFG was supported in part by an institutional grant from the Junta de Castilla y Le{\'o}n, Ref. CLU-2017-03 co-funded by the P.O. FEDER de Castilla y Le{\'o}n 14-20. This manuscript has been released as a preprint at bioRxiv (Gonz{\'a}lez-Arranz et al., 2020). Open Access publication fee is partially supported by the CSIC. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2020 Gonz{\'a}lez-Arranz, Gardner, Yu, Patel, Heldrich, Santos, Carballo, Jaspersen, Hochwagen and San-Segundo.",
year = "2020",
month = oct,
day = "22",
doi = "10.3389/fcell.2020.594092",
language = "English (US)",
volume = "8",
journal = "Frontiers in Cell and Developmental Biology",
issn = "2296-634X",
publisher = "Frontiers Media S. A.",
}