TY - JOUR
T1 - T cell hybridomas define the class II MHC-restricted response to vesicular stomatitis virus infection
AU - Petrarca, Michael A.
AU - Reiss, Carol S.
AU - Diamond, David C.
AU - Boni, Jurg
AU - Burakoff, Steven J.
AU - Faller, Douglas V.
PY - 1988/11
Y1 - 1988/11
N2 - T cell hybridomas with specificity for VSV (vesicular stomatitis virus)-infected cells were generated in an attempt to better define the la-restricted helper T cell response to VSV. The hybridomas were created by fusing BALB/c (H-2d) anti-VSV immune spleen cells to the murine thymoma BW 5147. These hybridomas produce IL-2 when stimulated with VSV-infected spleen cells. They were found to recognize viral antigens in association with I-Ad and, in addition, could also be stimulated by VSV-infected A20 cells (an la-positive B cell lymphoma of H-2d origin). The purified viral membrane glycoprotein, G protein, and Gs (secreted G protein that lacks the hydrophobic and intracytoplasmic domains) both stimulated IL-2 production when added to cultures of A20 and the hybridomas. These hybridomas therefore recognize a viral antigenic determinant on G protein. Since chemically-fixed antigen-presenting cells fail to stimulate the hybridomas after exogenous addition of purified G protein we can conclude that these T cell hybridomas recognize a processed form of the G protein. Stimulator cells created by expression in A20 of a transfected cDNA encoding G protein were also recognized. Recognition in this case was I-Ad-restricted, as anti-I-Ad monoclonal antibodies blocked stimulation, and an la-negative cell (P815) expressing a transfected G protein gene failed to stimulate the hybridomas. Even after paraformaldehyde fixation, G gene-transfected, la-positive cells could stimulate the hybridomas, suggesting that processing of this endogenously-synthesized antigen has occurred.
AB - T cell hybridomas with specificity for VSV (vesicular stomatitis virus)-infected cells were generated in an attempt to better define the la-restricted helper T cell response to VSV. The hybridomas were created by fusing BALB/c (H-2d) anti-VSV immune spleen cells to the murine thymoma BW 5147. These hybridomas produce IL-2 when stimulated with VSV-infected spleen cells. They were found to recognize viral antigens in association with I-Ad and, in addition, could also be stimulated by VSV-infected A20 cells (an la-positive B cell lymphoma of H-2d origin). The purified viral membrane glycoprotein, G protein, and Gs (secreted G protein that lacks the hydrophobic and intracytoplasmic domains) both stimulated IL-2 production when added to cultures of A20 and the hybridomas. These hybridomas therefore recognize a viral antigenic determinant on G protein. Since chemically-fixed antigen-presenting cells fail to stimulate the hybridomas after exogenous addition of purified G protein we can conclude that these T cell hybridomas recognize a processed form of the G protein. Stimulator cells created by expression in A20 of a transfected cDNA encoding G protein were also recognized. Recognition in this case was I-Ad-restricted, as anti-I-Ad monoclonal antibodies blocked stimulation, and an la-negative cell (P815) expressing a transfected G protein gene failed to stimulate the hybridomas. Even after paraformaldehyde fixation, G gene-transfected, la-positive cells could stimulate the hybridomas, suggesting that processing of this endogenously-synthesized antigen has occurred.
KW - T cell hybridoma
KW - antigen processing
KW - major histocompatibility complex
KW - vesicular stomatitis virus
KW - viral immunity
UR - http://www.scopus.com/inward/record.url?scp=0024241727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024241727&partnerID=8YFLogxK
U2 - 10.1016/0882-4010(88)90033-2
DO - 10.1016/0882-4010(88)90033-2
M3 - Article
C2 - 2853279
AN - SCOPUS:0024241727
VL - 5
SP - 319
EP - 332
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
SN - 0882-4010
IS - 5
ER -