Tag games in yeast: The two-hybrid system and beyond

Rainer K. Brachmann, Jef D. Boeke

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The yeast Saccharomyces cerevisiae and the one- and two-hybrid systems are essential genetic tools for studying the macromolecular interactions that define all living organisms. Newly developed variations on this theme can now address an even bigger set of questions. Reverse one- and two-hybrid systems can identify factors that dissociate or abrogate defined macromolecular interactions. Different forms of three-hybrid systems can evaluate the complex interplay of proteins with RNAs, peptide ligands, small organic ligands or protein kinases. Finally, the ubiquitin-based split-protein sensor and the Sos recruitment systems promise to overcome some limitations of conventional two-hybrid systems.

    Original languageEnglish (US)
    Pages (from-to)561-568
    Number of pages8
    JournalCurrent Opinion in Biotechnology
    Volume8
    Issue number5
    DOIs
    StatePublished - Oct 1997

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering

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