TY - JOUR
T1 - Tag games in yeast
T2 - The two-hybrid system and beyond
AU - Brachmann, Rainer K.
AU - Boeke, Jef D.
PY - 1997/10
Y1 - 1997/10
N2 - The yeast Saccharomyces cerevisiae and the one- and two-hybrid systems are essential genetic tools for studying the macromolecular interactions that define all living organisms. Newly developed variations on this theme can now address an even bigger set of questions. Reverse one- and two-hybrid systems can identify factors that dissociate or abrogate defined macromolecular interactions. Different forms of three-hybrid systems can evaluate the complex interplay of proteins with RNAs, peptide ligands, small organic ligands or protein kinases. Finally, the ubiquitin-based split-protein sensor and the Sos recruitment systems promise to overcome some limitations of conventional two-hybrid systems.
AB - The yeast Saccharomyces cerevisiae and the one- and two-hybrid systems are essential genetic tools for studying the macromolecular interactions that define all living organisms. Newly developed variations on this theme can now address an even bigger set of questions. Reverse one- and two-hybrid systems can identify factors that dissociate or abrogate defined macromolecular interactions. Different forms of three-hybrid systems can evaluate the complex interplay of proteins with RNAs, peptide ligands, small organic ligands or protein kinases. Finally, the ubiquitin-based split-protein sensor and the Sos recruitment systems promise to overcome some limitations of conventional two-hybrid systems.
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U2 - 10.1016/S0958-1669(97)80029-8
DO - 10.1016/S0958-1669(97)80029-8
M3 - Article
C2 - 9353226
AN - SCOPUS:0030690331
SN - 0958-1669
VL - 8
SP - 561
EP - 568
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 5
ER -