Tapping mode atomic force microscopy of hyaluronan: Extended and intramolecularly interacting chains

Mary K. Cowman; Min Li; Endre A. Balazs

doi:10.1016/S0006-3495(98)77645-3

Tapping mode atomic force microscopy of hyaluronan: Extended and intramolecularly interacting chains

Mary K. Cowman, Min Li, Endre A. Balazs

Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

The extracellular matrix polysaccharide hyaluronan has been examined by tapping mode atomic force microscopy. High molecular weight hyaluronan was deposited on mica from dilute aqueous solution and imaged in air. Long unbranched chains could be observed and were found to be compatible with the known covalent structure of hyaluronan. In addition, chains with evidence of intramolecular association were observed. In the simplest cases, the association took the form of loops stabilized by antiparallel double-stranded (probably double-helical) segments. In other cases, the polarity of the associated regions could not be determined. Extensive intramolecular association in long hyaluronan chains resulted in a fenestrated structure of the same type as that formed by intermolecular association at higher concentrations.

Original language	English (US)
Pages (from-to)	2030-2037
Number of pages	8
Journal	Biophysical journal
Volume	75
Issue number	4
DOIs	https://doi.org/10.1016/S0006-3495(98)77645-3
State	Published - Oct 1998

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(98)77645-3

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title = "Tapping mode atomic force microscopy of hyaluronan: Extended and intramolecularly interacting chains",

abstract = "The extracellular matrix polysaccharide hyaluronan has been examined by tapping mode atomic force microscopy. High molecular weight hyaluronan was deposited on mica from dilute aqueous solution and imaged in air. Long unbranched chains could be observed and were found to be compatible with the known covalent structure of hyaluronan. In addition, chains with evidence of intramolecular association were observed. In the simplest cases, the association took the form of loops stabilized by antiparallel double-stranded (probably double-helical) segments. In other cases, the polarity of the associated regions could not be determined. Extensive intramolecular association in long hyaluronan chains resulted in a fenestrated structure of the same type as that formed by intermolecular association at higher concentrations.",

author = "Cowman, {Mary K.} and Min Li and Balazs, {Endre A.}",

note = "Funding Information: This research was supported by Biomatrix, Inc. This work was presented at the 1998 annual meeting of the Biophysical Society in Kansas City, MO. ",

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language = "English (US)",

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T1 - Tapping mode atomic force microscopy of hyaluronan

T2 - Extended and intramolecularly interacting chains

AU - Cowman, Mary K.

AU - Li, Min

AU - Balazs, Endre A.

N1 - Funding Information: This research was supported by Biomatrix, Inc. This work was presented at the 1998 annual meeting of the Biophysical Society in Kansas City, MO.

PY - 1998/10

Y1 - 1998/10

N2 - The extracellular matrix polysaccharide hyaluronan has been examined by tapping mode atomic force microscopy. High molecular weight hyaluronan was deposited on mica from dilute aqueous solution and imaged in air. Long unbranched chains could be observed and were found to be compatible with the known covalent structure of hyaluronan. In addition, chains with evidence of intramolecular association were observed. In the simplest cases, the association took the form of loops stabilized by antiparallel double-stranded (probably double-helical) segments. In other cases, the polarity of the associated regions could not be determined. Extensive intramolecular association in long hyaluronan chains resulted in a fenestrated structure of the same type as that formed by intermolecular association at higher concentrations.

AB - The extracellular matrix polysaccharide hyaluronan has been examined by tapping mode atomic force microscopy. High molecular weight hyaluronan was deposited on mica from dilute aqueous solution and imaged in air. Long unbranched chains could be observed and were found to be compatible with the known covalent structure of hyaluronan. In addition, chains with evidence of intramolecular association were observed. In the simplest cases, the association took the form of loops stabilized by antiparallel double-stranded (probably double-helical) segments. In other cases, the polarity of the associated regions could not be determined. Extensive intramolecular association in long hyaluronan chains resulted in a fenestrated structure of the same type as that formed by intermolecular association at higher concentrations.

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JO - Biophysical journal

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Tapping mode atomic force microscopy of hyaluronan: Extended and intramolecularly interacting chains

Abstract

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