Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly

Steven Isaacman; Michael Buckley; Xiaojian Wang; Edwin Y. Wang; Leonard Liebes; James W. Canary

doi:10.1016/j.bmcl.2014.01.063

Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly

Steven Isaacman, Michael Buckley, Xiaojian Wang, Edwin Y. Wang, Leonard Liebes, James W. Canary

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Nanometer-scale architectures assembled on cell surface receptors from smaller macromolecular constituents generated a large amplification of fluorescence. A targeted dendrimer was synthesized from a cystamine-core G4 PAMAM dendrimer, and contained an anti-BrE3 monoclonal antibody as the targeting group, several fluorophores and an average of 12 aldehyde moieties as complementary bio-orthogonal reactive sites for the covalent assembly. A cargo dendrimer, derived from a PAMAM G4 dendrimer, contained several fluorophores as the cargo for delivery and five hydrazine moieties as complimentary bio-orthogonal reactive sites. The system is designed to be flexible and allow for facile incorporation of a variety of targeting ligands.

Original language	English (US)
Pages (from-to)	1290-1293
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	24
Issue number	5
DOIs	https://doi.org/10.1016/j.bmcl.2014.01.063
State	Published - Mar 1 2014

Keywords

Cell surface self-assembly
Dendrimer
Targeted amplification

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/j.bmcl.2014.01.063

Cite this

@article{d009124b3cbf4a16991a75bf52d2d9b4,

title = "Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly",

abstract = "Nanometer-scale architectures assembled on cell surface receptors from smaller macromolecular constituents generated a large amplification of fluorescence. A targeted dendrimer was synthesized from a cystamine-core G4 PAMAM dendrimer, and contained an anti-BrE3 monoclonal antibody as the targeting group, several fluorophores and an average of 12 aldehyde moieties as complementary bio-orthogonal reactive sites for the covalent assembly. A cargo dendrimer, derived from a PAMAM G4 dendrimer, contained several fluorophores as the cargo for delivery and five hydrazine moieties as complimentary bio-orthogonal reactive sites. The system is designed to be flexible and allow for facile incorporation of a variety of targeting ligands.",

keywords = "Cell surface self-assembly, Dendrimer, Targeted amplification",

author = "Steven Isaacman and Michael Buckley and Xiaojian Wang and Wang, {Edwin Y.} and Leonard Liebes and Canary, {James W.}",

note = "Funding Information: We are grateful to Peter Brooks (Maine Medical Research Institute) for helpful discussions. We thank the NSF (ODISSEI-1332411), the NYU Cancer Institute, the NYU Department of Radiology, and the American Society for Neuroradiology for financial support. ",

year = "2014",

month = mar,

day = "1",

doi = "10.1016/j.bmcl.2014.01.063",

language = "English (US)",

volume = "24",

pages = "1290--1293",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd",

number = "5",

}

TY - JOUR

T1 - Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly

AU - Isaacman, Steven

AU - Buckley, Michael

AU - Wang, Xiaojian

AU - Wang, Edwin Y.

AU - Liebes, Leonard

AU - Canary, James W.

N1 - Funding Information: We are grateful to Peter Brooks (Maine Medical Research Institute) for helpful discussions. We thank the NSF (ODISSEI-1332411), the NYU Cancer Institute, the NYU Department of Radiology, and the American Society for Neuroradiology for financial support.

PY - 2014/3/1

Y1 - 2014/3/1

N2 - Nanometer-scale architectures assembled on cell surface receptors from smaller macromolecular constituents generated a large amplification of fluorescence. A targeted dendrimer was synthesized from a cystamine-core G4 PAMAM dendrimer, and contained an anti-BrE3 monoclonal antibody as the targeting group, several fluorophores and an average of 12 aldehyde moieties as complementary bio-orthogonal reactive sites for the covalent assembly. A cargo dendrimer, derived from a PAMAM G4 dendrimer, contained several fluorophores as the cargo for delivery and five hydrazine moieties as complimentary bio-orthogonal reactive sites. The system is designed to be flexible and allow for facile incorporation of a variety of targeting ligands.

AB - Nanometer-scale architectures assembled on cell surface receptors from smaller macromolecular constituents generated a large amplification of fluorescence. A targeted dendrimer was synthesized from a cystamine-core G4 PAMAM dendrimer, and contained an anti-BrE3 monoclonal antibody as the targeting group, several fluorophores and an average of 12 aldehyde moieties as complementary bio-orthogonal reactive sites for the covalent assembly. A cargo dendrimer, derived from a PAMAM G4 dendrimer, contained several fluorophores as the cargo for delivery and five hydrazine moieties as complimentary bio-orthogonal reactive sites. The system is designed to be flexible and allow for facile incorporation of a variety of targeting ligands.

KW - Cell surface self-assembly

KW - Dendrimer

KW - Targeted amplification

UR - http://www.scopus.com/inward/record.url?scp=84894531318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894531318&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2014.01.063

DO - 10.1016/j.bmcl.2014.01.063

M3 - Article

C2 - 24513050

AN - SCOPUS:84894531318

SN - 0960-894X

VL - 24

SP - 1290

EP - 1293

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 5

ER -

Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this