Templated DNA ligation with thiol chemistry

Dadong Li; Xiaojian Wang; Fubo Shi; Ruojie Sha; Nadrian C. Seeman; James W. Canary

doi:10.1039/c4ob01552e

Templated DNA ligation with thiol chemistry

Dadong Li, Xiaojian Wang, Fubo Shi, Ruojie Sha, Nadrian C. Seeman, James W. Canary

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.

Original language	English (US)
Pages (from-to)	8823-8827
Number of pages	5
Journal	Organic and Biomolecular Chemistry
Volume	12
Issue number	44
DOIs	https://doi.org/10.1039/c4ob01552e
State	Published - Nov 28 2014

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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abstract = "We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.",

author = "Dadong Li and Xiaojian Wang and Fubo Shi and Ruojie Sha and Seeman, {Nadrian C.} and Canary, {James W.}",

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AU - Li, Dadong

AU - Wang, Xiaojian

AU - Shi, Fubo

AU - Sha, Ruojie

AU - Seeman, Nadrian C.

AU - Canary, James W.

N1 - Publisher Copyright: © the Partner Organisations 2014.

PY - 2014/11/28

Y1 - 2014/11/28

N2 - We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.

AB - We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.

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JO - Organic and Biomolecular Chemistry

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ER -

Templated DNA ligation with thiol chemistry

Abstract

ASJC Scopus subject areas

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