We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.
|Original language||English (US)|
|Number of pages||5|
|Journal||Organic and Biomolecular Chemistry|
|State||Published - Nov 28 2014|
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry