Templated DNA ligation with thiol chemistry

Dadong Li, Xiaojian Wang, Fubo Shi, Ruojie Sha, Nadrian C. Seeman, James W. Canary

Research output: Contribution to journalArticlepeer-review


We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.

Original languageEnglish (US)
Pages (from-to)8823-8827
Number of pages5
JournalOrganic and Biomolecular Chemistry
Issue number44
StatePublished - Nov 28 2014

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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