TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs

Nurjana Bachman, Marnie E. Gelbart, Toshio Tsukiyama, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    Retrotransposons are RNA elements that reverse transcribe their RNA genomes and make a cDNA copy that is inserted back into a new genomic location by the element-encoded integrase protein. Ty1 is a long terminal repeat (LTR) retrotransposon in Saccharomyces cerevisiae that inserts into an ∼700-bp integration window upstream of tRNA genes with a periodicity of ∼80 bp. ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection. We show that the N terminus of Bdp1p, a component of the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into the integration window. Deletion of the Bdp1p N terminus and mutation of ISW2 result in similar disruption of nucleosome positioning upstream of some tRNA genes, and the N-terminal domain of Bdp1p is required for targeting of Isw2 complex to tRNA genes. This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo.

    Original languageEnglish (US)
    Pages (from-to)955-964
    Number of pages10
    JournalGenes and Development
    Volume19
    Issue number8
    DOIs
    StatePublished - Apr 15 2005

    Keywords

    • Chromatin
    • Integrase
    • RNA polymerase III
    • Retrotransposon
    • Transcription factor
    • tRNA gene

    ASJC Scopus subject areas

    • Genetics
    • Developmental Biology

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