Abstract
Retrotransposons are RNA elements that reverse transcribe their RNA genomes and make a cDNA copy that is inserted back into a new genomic location by the element-encoded integrase protein. Ty1 is a long terminal repeat (LTR) retrotransposon in Saccharomyces cerevisiae that inserts into an ∼700-bp integration window upstream of tRNA genes with a periodicity of ∼80 bp. ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection. We show that the N terminus of Bdp1p, a component of the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into the integration window. Deletion of the Bdp1p N terminus and mutation of ISW2 result in similar disruption of nucleosome positioning upstream of some tRNA genes, and the N-terminal domain of Bdp1p is required for targeting of Isw2 complex to tRNA genes. This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo.
Original language | English (US) |
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Pages (from-to) | 955-964 |
Number of pages | 10 |
Journal | Genes and Development |
Volume | 19 |
Issue number | 8 |
DOIs | |
State | Published - Apr 15 2005 |
Keywords
- Chromatin
- Integrase
- RNA polymerase III
- Retrotransposon
- Transcription factor
- tRNA gene
ASJC Scopus subject areas
- General Medicine