Purpose: CLL studies have shown that CLL cells and normal lymphocytes flow equally through Millipore filters, but CLL cells adhere more to the TM. This study attempts to compare the TM adhesion of normal granulocytes, lymphocytes, and the abnormal cells of the AML, CML, and ALL. The results will then be compared to the CLL results obtained from the previous experiments. Methods: Normal granulocytes, lymphocytes, AML, CML, and ALL cells were all collected from the peripheral blood of volunteers. Histopaque-1077 and Histopaque -1119 gradient centrifugation was used to isolate cells. Cells were then cultured in RPMI -1640 medium. They were stained with fluorescein diacetate and layered onto frozen sections of TM. The specimens were then incubated at 37 deg C for 40 minutes and washed twice with phosphate buffered saline. Fluorescence microscopy was used to count the cells in the TM. Each experiment was done 3 times. N value for AML = 94, normal granulocytes n=99, CML n=95, normal granulocytes n=100, ALL n=99, normal lymphocytes n=98, CLL n=8, normal lymphocytes n=10. Results: There were significantly more AML and CML cells adherent to the TM than normal granulocytes (p=000 for both groups). When AML and CML were compared to CLL cells, CLL cells appeared to adhere less than both AML and CML (p=0000). No significant difference was observed between normal lymphocytes and ALL cells (p=0.4079). Conclusions: Normal lymphocytes and ALL cells appeared to adhere equally to the TM. AML and CML findings are consistent with the previous CLL observations and further support the hypothesis that leukemic cells decrease the outflow facility and increase the IOP via biological interaction rather than pure mechanical obstruction. Leukocyte adhesion molecules (CAMs) such as LFA-1 might account for the increased adhesiveness by interacting with CAMs such as ICAM, which is reported to be present in the TM.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Feb 15 1996|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience