TY - JOUR
T1 - The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells
T2 - A comparative assessment of Colombian samples
AU - Mora, Dolly Patricia Pardo
AU - Santiago, Karina Basso
AU - Conti, Bruno José
AU - de Oliveira Cardoso, Eliza
AU - Conte, Fernanda Lopes
AU - Oliveira, Lucas Pires Garcia
AU - de Assis Golim, Marjorie
AU - Uribe, Jaime Fabian Cruz
AU - Gutiérrez, Rafael María
AU - Buitrago, Mauricio Rey
AU - Popova, Milena
AU - Trusheva, Boryana
AU - Bankova, Vassya
AU - García, Orlando Torres
AU - Sforcin, José Maurício
N1 - Publisher Copyright:
© 2018 John Wiley & Sons, Ltd.
PY - 2019/3
Y1 - 2019/3
N2 - Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC 50 ). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH-DA. Transwell assay was used to evaluate the invasiveness of propolis-treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.
AB - Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC 50 ). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH-DA. Transwell assay was used to evaluate the invasiveness of propolis-treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.
KW - apoptosis
KW - chemical composition
KW - cytotoxicity
KW - mitochondrial membrane potential
KW - osteosarcoma
KW - propolis
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U2 - 10.1002/ptr.6246
DO - 10.1002/ptr.6246
M3 - Article
C2 - 30488503
AN - SCOPUS:85057528291
SN - 0951-418X
VL - 33
SP - 591
EP - 601
JO - Phytotherapy Research
JF - Phytotherapy Research
IS - 3
ER -