The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples

Dolly Patricia Pardo Mora, Karina Basso Santiago, Bruno José Conti, Eliza de Oliveira Cardoso, Fernanda Lopes Conte, Lucas Pires Garcia Oliveira, Marjorie de Assis Golim, Jaime Fabian Cruz Uribe, Rafael María Gutiérrez, Mauricio Rey Buitrago, Milena Popova, Boryana Trusheva, Vassya Bankova, Orlando Torres García, José Maurício Sforcin

Research output: Contribution to journalArticle

Abstract

Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC 50 ). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH-DA. Transwell assay was used to evaluate the invasiveness of propolis-treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.

Original languageEnglish (US)
Pages (from-to)591-601
Number of pages11
JournalPhytotherapy Research
Volume33
Issue number3
DOIs
StatePublished - Mar 2019

Keywords

  • apoptosis
  • chemical composition
  • cytotoxicity
  • mitochondrial membrane potential
  • osteosarcoma
  • propolis

ASJC Scopus subject areas

  • Pharmacology

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  • Cite this

    Mora, D. P. P., Santiago, K. B., Conti, B. J., de Oliveira Cardoso, E., Conte, F. L., Oliveira, L. P. G., de Assis Golim, M., Uribe, J. F. C., Gutiérrez, R. M., Buitrago, M. R., Popova, M., Trusheva, B., Bankova, V., García, O. T., & Sforcin, J. M. (2019). The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples. Phytotherapy Research, 33(3), 591-601. https://doi.org/10.1002/ptr.6246