The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription

Piergiorgio Percipalle; Nathalie Fomproix; Erica Cavellán; Renate Voit; Georg Reimer; Tim Krüger; Johan Thyberg; Ulrich Scheer; Ingrid Grummt; Ann Kristin Östlund Farrants

doi:10.1038/sj.embor.7400657

The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription

Piergiorgio Percipalle, Nathalie Fomproix, Erica Cavellán, Renate Voit, Georg Reimer, Tim Krüger, Johan Thyberg, Ulrich Scheer, Ingrid Grummt, Ann Kristin Östlund Farrants

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein - protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.

Original language	English (US)
Pages (from-to)	525-530
Number of pages	6
Journal	EMBO Reports
Volume	7
Issue number	5
DOIs	https://doi.org/10.1038/sj.embor.7400657
State	Published - May 2006

Keywords

Chromatin remodelling complexes
Nuclear myosin 1
Pol I transcription
SNF2h
WSTF
rDNA

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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title = "The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription",

abstract = "Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein - protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.",

keywords = "Chromatin remodelling complexes, Nuclear myosin 1, Pol I transcription, SNF2h, WSTF, rDNA",

author = "Piergiorgio Percipalle and Nathalie Fomproix and Erica Cavell{\'a}n and Renate Voit and Georg Reimer and Tim Kr{\"u}ger and Johan Thyberg and Ulrich Scheer and Ingrid Grummt and {{\"O}stlund Farrants}, {Ann Kristin}",

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doi = "10.1038/sj.embor.7400657",

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T1 - The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription

AU - Percipalle, Piergiorgio

AU - Fomproix, Nathalie

AU - Cavellán, Erica

AU - Voit, Renate

AU - Reimer, Georg

AU - Krüger, Tim

AU - Thyberg, Johan

AU - Scheer, Ulrich

AU - Grummt, Ingrid

AU - Östlund Farrants, Ann Kristin

PY - 2006/5

Y1 - 2006/5

N2 - Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein - protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.

AB - Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein - protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.

KW - Chromatin remodelling complexes

KW - Nuclear myosin 1

KW - Pol I transcription

KW - SNF2h

KW - WSTF

KW - rDNA

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JF - EMBO Reports

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The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription

Abstract

Keywords

ASJC Scopus subject areas

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