The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription

Piergiorgio Percipalle, Nathalie Fomproix, Erica Cavellán, Renate Voit, Georg Reimer, Tim Krüger, Johan Thyberg, Ulrich Scheer, Ingrid Grummt, Ann Kristin Östlund Farrants

Research output: Contribution to journalArticle

Abstract

Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein - protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.

Original languageEnglish (US)
Pages (from-to)525-530
Number of pages6
JournalEMBO Reports
Volume7
Issue number5
DOIs
StatePublished - May 2006

Keywords

  • Chromatin remodelling complexes
  • Nuclear myosin 1
  • Pol I transcription
  • SNF2h
  • WSTF
  • rDNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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