The conserved NPX(n)Y motif present in the gastrin-releasing peptide receptor is not a general sequestration sequence

L. W. Slice, H. C. Wong, C. Sternini, E. F. Grady, N. W. Bunnett, J. H. Walsh

Research output: Contribution to journalArticlepeer-review

Abstract

Exposure of the gastrin-releasing peptide (GRP) receptor to agonists causes a rapid desensitization of the receptor-stimulated mobilization of intracellular calcium. Homologous desensitization occurs by uncoupling the G- proteins from the receptor and by ligand induced internalization. The molecular determinants of desensitization of the GRP receptor are not well known. The importance of tyrosine 324 which is located in a highly conserved NPX2-3Y motif of the GRP receptor was investigated. Kirsten murine sarcoma virus-transformed rat kidney (KNRK) cells were transfected with expression vectors encoding either the wild type or the mutant (tyrosine 324 to alanine 324) rat GRP receptor. The wild type and mutant GRP receptors were expressed at a high level in the KNRK cells, 2.0 x 106 and 0.5 x 106 receptors per cell, respectively. The wild type and mutant GRP receptors bound GRP with the same affinity (K(d) = 6-7 nM). KNRK cells expressing the wild type or mutant GRP receptor had similar [Ca2+](i) dose response to GRP. KNRK cells expressing the GRP receptor rapidly internalized bound 125I-GRP at 37 °C. Internalization was inhibited at 4 °C and by 0.45 M sucrose. The internalization of bound 125I-GRP by the mutant GRP receptor was identical to the wild type receptor. Fluorescent microscopy was used to directly observe the GRP receptor expressed on the surface of the KNRK cells and to visualize its ligand induced internalization. There was no difference in the pattern of internalization between the wild type and mutant GRP receptors expressed in KNRK cells. Therefore, the highly conserved tyrosine 324 does not have a role in GRP binding, receptor-G-protein interaction, or initial events of ligand induced receptor internalization. The NPX(n)Y motif is not a general sequestration sequence for seven transmembrane G-protein linked receptors.

Original languageEnglish (US)
Pages (from-to)21755-21761
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number34
StatePublished - 1994

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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