Abstract
In order to assess blood pressure control drugs, the endothelial cellular biosensing system for assessing blood pressure control drugs was constructed. This system consists of human umbilical vein endothelial cells (HUVEC) on a polyion-coated gold electrode, a platinum counter electrode and an Ag/AgCl reference electrode. Nitric oxide (NO) as an indicator of blood vessel relaxation was detected with a polyion-coated electrode in the system. The NO detection limit of this electrode was 8.4nM by differential pulse voltammetry (DPV). The drugs of blood pressure control (acetylcholine chloride (AcChCl), NOC 7 and NG-monomethyl-L-arginine (L-NMMA)) were assessed with this endothelial cellular biosensing system. One milli molar of AcChCl make NO released from HUVEC stimulated by activating endothelial nitric oxide synthase (eNOS) in HUVEC. In the case of 5mM of L-NMMA, NO releasing was inhibited by inhibiting eNOS activation by 1mM of AcChCl. NOC 7 immediately released NO regardless of eNOS activation in endothelial cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1121-1124 |
| Number of pages | 4 |
| Journal | Biosensors and Bioelectronics |
| Volume | 19 |
| Issue number | 9 |
| DOIs | |
| State | Published - Apr 15 2004 |
Keywords
- Acetylcholine
- Blood pressure
- Cellular biosensing system
- Endothelial cell
- L-NMMA
- Nitric oxide
- NOC 7
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry